Authors: Naling Bai Sheng Wang Rexiding Abuduaini Xufen Zhu Yuhua Zhao
Publish Date: 2016/03/10
Volume: 23, Issue: 12, Pages: 12019-12029
Abstract
Nonylphenol polyethoxylates NPEOs although banned for decades are still widely used in manufactories and thus affect human lives In this study a highly efficient NPEOdegrading bacterium Sphingomonas sp Y2 was isolated from sewage sludge by enrichment culture Strain Y2 ensured the complete removal of NPEO in 48 h and degraded 992 NPEO 1000 mg L−1 within 30 h at a specific growth rate of 073 h−1 in minimum salt medium To date this degradation efficiency is the highest reported for NPEO metabolism by a pure bacterium under this condition Furthermore the application of this bacterium to wastewater treatment demonstrated that it metabolized 985 NPEO 1000 mg L−1 within 5 days with a specific growth rate of 203 day−1 The degradation intermediates identified as nonylphenol shortchain NPEOs and shortchain nonylphenol polyethoxycarboxylates by highperformance liquid chromatography and gas chromatographymass spectrometry indicated the sequential exocleavage of the EO chain Additionally the enzymes involved in the biodegradation were inducible rather than constitutive Considering that strain Y2 exhibits prominent biodegradation advantages in industrial wastewater treatment it might serve as a promising potential candidate for in situ bioremediation of contamination by NPEOs and other structurally similar compoundsThis study was supported by the National Natural Science Foundation of China 41271335 31470191 the Major State Basic Research Development Program of China 973 program 2015CB150502 the High Technology Research and Development Program of China 863 Program 2012AA06A203 and the National Key Technology Rand D Program 2012BAC17B04 the Science and Technology Project of Zhejiang Province 2013C3303 2014C33019 and the Environmental Science Project of Zhejiang Province 2012B006
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