Authors: Mingyang Jing Yang Liu Wei Song Yunxing Yan Wenbao Yan Rutao Liu
Publish Date: 2015/09/11
Volume: 23, Issue: 2, Pages: 1335-1343
Abstract
Copper can disturb the intracellular redox balance induce oxidative stress and subsequently cause irreversible damage leading to a variety of diseases In the present study mouse primary hepatocytes were chosen to elucidate the in vitro oxidative damage of shortterm copper exposure 10–200 μM by singlecell analysis We evaluated the toxicity of copper by reactive oxygen species ROS glutathione GSH and oxidative DNA damage at the singlecell level Oxidative damage induced by copper was verified by the morphological changes persistent elevations of excessive ROS and malondialdehyde MDA a decrease in GSH level and the oxidative DNA damage Furthermore the average ROS generation GSH consumption and the indicators in DNA damage did not significantly change at relatively low concentrations 10 or 50 μM but we can find the alterations of parameters in some single cells clearly Emphasis on the analysis of single cells is conducive to gain a better understanding on the toxicity of copper This study will also complement studies on the environmental risk assessment of copper pollutionThis work is supported by NSFC 20875055 21277081 201477067 the Cultivation Fund of the Key Scientific and Technical Innovation Project Research Fund for the Doctoral Program of Higher Education and Ministry of Education of China 708058 20130131110016 and Independent Innovation Foundation of Shandong University natural science projects 2012DX002 is also acknowledged
Keywords: