Authors: Yuexia Zhang Zhenhua Yang Yan Feng Ruijin Li Quanxi Zhang Hong Geng Chuan Dong
Publish Date: 2015/04/24
Volume: 22, Issue: 16, Pages: 12450-12457
Abstract
The main aim of the present study was to examine in vitro responses of rat alveolar macrophages AMs exposed to coarse chalk dust particles particulate matter in the size range 25–10 μm PMcoarse by respiratory burst and oxidative stress Chalk PMcoarseinduced respiratory burst in AMs was measured by using a luminoldependent chemiluminescence CL method Also the cell viability lactate dehydrogenase LDH release levels of cellular superoxide dismutase SOD catalase CAT glutathione GSH malondialdehyde MDA and acid phosphatase ACP plasma membrane ATPase and extracellular nitric oxide NO level were determined 4 h following the treatment with the different dosages of chalk PMcoarse The results showed that chalk PMcoarse initiated the respiratory burst of AMs as indicated by strong CL which was inhibited by diphenyleneiodonium chloride and lNnitrolarginine methyl ester hydrochloride It suggested that chalk PMcoarse induced the production of reactive oxygen species ROS and reactive nitrogen species RNS in AMs This hypothesis was confirmed by the fact that chalk PMcoarse resulted in a significant decrease of intracellular SOD GSH ACP and ATPase levels and a notable increase of intracellular CAT MDA content and extracellular NO level consequently leading to a decrease of the cell viability and a increase of LDH release It was concluded that AMs exposed to chalk PMcoarse can suffer from cytotoxicity which may be mediated by generation of excessive ROS/RNS
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