Authors: Thomas E Schumacher Anna Eynard Rajesh Chintala
Publish Date: 2014/12/04
Volume: 22, Issue: 6, Pages: 4759-4762
Abstract
Fluorescein diacetate FDA is commonly used to determine the hydrolyzing activity of microbial organisms in the soil However the costs of chemical reagents and time required to perform routine analysis of large number of samples by soil testing laboratories are limiting Moreover existing methods generate significant volumes of hazardous waste In this context this study was designed to determine the minimum amount of terminating chemical reagent needed to evaluate microbial hydrolyzing activity The results showed that 02 mL of chloroform was enough to effectively stop the hydrolyzing activity in soil This proposed terminating chemical reagent 02 mL chloroform was also evaluated by comparing with the 10 mL of chloroform and 5 mL of methanol used in the Adam and Duncan method
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