Authors: Fatma Zohra Mesbaiah Kamel Eddouaouda Abdelmalek Badis Alif Chebbi Dorra Hentati Sami Sayadi Mohamed Chamkha
Publish Date: 2016/04/07
Volume: 23, Issue: 14, Pages: 14221-14230
Abstract
The capacities of a biosurfactant producing and polycyclic aromatic hydrocarbon PAH utilizing bacterium namely strain 1C isolated from an Algerian contaminated soil were investigated Strain 1C belonged to the Paenibacillus genus and was closely related to the specie Paenibacillus popilliae with 16S rRNA gene sequence similarity of 984 It was able to produce biosurfactant using olive oil as substrate The biosurfactant production was shown by surface tension 326 mN/m after 24 h of incubation at 45 °C and 150 rpm The biosurfactants retained its properties during exposure to elevated temperatures 70 °C relatively high salinity 20 NaCl and a wide range of pH values 2–10 The infrared spectroscopy FTIR revealed that its chemical structure belonged to lipopeptide class The critical micelle concentration CMC of this biosurfactant was about 05 g/l with 294 mN/m In addition the surface active compounds produced by strain 1C enhanced PAH solubility and showed a significant antimicrobial activity against pathogens In addition to its biosurfactant production strain 1C was shown to be able to utilize PAHs as the sole carbon and energy sources Strain 1C as hydrocarbonoclastic bacteria and its interesting surface active agent may be used for cleaning the environments polluted with polyaromatic hydrocarbonsThis work was funded by the Algerian Ministry of Higher Education and Scientific Research by the CNEPRU project number J0100420120004 The authors would like to express their gratitude to Mr Anouar Smaoui from the English Language Unit at the Faculty of Science of Sfax Tunisia for his valuable proofreading and languagepolishing services We express our thanks to Mr Nidhal Bakkar and Mr Hocine Boutoumi for their assistance in IR analyses and Mrs Najla Mhiri and Mrs Manel Chelbi for their help with molecular techniques
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