Authors: Mingqin Zhong Pinghe Yin Ling Zhao
Publish Date: 2017/03/10
Volume: 24, Issue: 11, Pages: 10519-10527
Abstract
The objective of the present work was to evaluate the toxic effect of nonylphenol NP on the antioxidant response and antitumor activity of Gracilaria lemaneiformis An obvious oxidative damage was observed in this study The thallus exposed to NP showed 12–20fold increase in lipid peroxide and displayed a maximum level of 1658 μmol g−1 Fw on 06 mg L−1 for 15day exposure The activities of antioxidant enzymes such as superoxide dismutase SOD and catalase CAT enhanced significantly by 11–32fold and subsequently diminished at the high concentrations and prolonged exposure The results of DNA damage in comet assay also supported that NP was obviously toxic on G lemaneiformis with increasing the percentage of tail DNA in a dosedependent manner Furthermore the ethanol extract of G lemaneiformis EEGL did exhibit antitumor potential against HepG2 cells While decreased in cell inhibition ROS generation apoptosis and caspase3 in HepG2 cells treated with the EEGL were observed when G lemaneiformis was exposed to NP for 15 days and which were related to exposure concentration of NP These suggested that NP has strongly toxic effect on the antitumor activity of G lemaneiformis The results revealed in this study imply that macroalgae can be useful biomarkers to evaluate marine pollutionsThis study was financially supported by the NSFCGuangdong Province Joint Key Project U1301235 IndustryUniversityResearch Combination Project of Guangdong Province 2013B090600009 Science and Technology Program of Guangdong China 2014A020217007 and National Natural Science Foundation of China 41676110
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