Authors: Xiaohong Chen Zhiqiang Cai
Publish Date: 2015/10/21
Volume: 23, Issue: 4, Pages: 3517-3524
Abstract
ZJ0273 propyl 424 6demethoxy pyrimidin2yloxy benzylamino benzoate is a novel pyrimidynyloxybenzoicbased herbicide developed in China for oilseed crop This study was aimed to construct new strains capable of degrading naphthalene and ZJ0273 by protoplast fusion between Amycolatopsis sp M31 and Pseudomonas sp Nai8 Eight recombinant strains were successfully produced and the strains could simultaneously utilize ZJ0273 and naphthalene as the sole carbon and energy source respectively One of recombinant strains MN6 with higher degrading efficiency was chosen for further study Under the condition of pH 70 30 °C ZJ0273 and naphthalene degradation percent by the recombinant strain MN6 could reach 6510 20 days and 8846 48 h respectively According to the identified six metabolites M1–M6 by LC–MS/MS biodegradation pathway of ZJ0273 was proposed ZJ0273 biodegradation catalyzed by the recombinant strain MN6 involved continuous biocatalytic reactions such as deestering hydrolysis acylation CN cleavage demethyl and ether cleavage reactions
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