Authors: Nilesh S Gangurde Riyaz Z Sayyed Shashi Kiran Arvind Gulati
Publish Date: 2012/06/22
Volume: 20, Issue: 1, Pages: 488-497
Abstract
During screening for polyβhydroxybutyrate PHB producing bacteria from distillery effluent sample six out of 30 isolates comprising of three strains of Alcaligenes sp two strains of Bacillus sp and one strain of Pseudomonas sp were found to accumulate varying levels of intracellular PHB Amongst the various isolates Alcaligenes sp RZS4 was found as the potent PHBproducing organism accumulating higher amounts of PHB PHB productivity was further enhanced in the presence of oxygen nitrogenlimiting conditions and cloning of PHB synthesizing genes of Alcaligenes sp RZS 4 into Escherichia coli A twofold increase in PHB yield was obtained from recombinant E coli visàvis Alcaligenes sp the recombinant E coli accumulated more PHB in NDMM produced good amount of PHB in a singlestage cultivation process under both nutrientrich and nutrientdeficient conditions Extraction of PHB with acetone–alcohol 11 was found as suitable method for optimum extraction of PHB as this mixture selectively extracted PHB without affecting the nonPHB cell mass PHB extract from recombinant E coli showed the presence of C–H =O stretching =C–H deformation =C–H =CH and =C–O functional groups characteristic of PHBAuthor NSG is thankful to the Council of Scientific and Industrial Research CSIR Government of India New Delhi for SRF and RZS is thankful to the Department of Science and Technology DST Government of India New Delhi for the financial assistance AG and SK acknowledge the Director CSIRInstitute of Himalayan Bioresource Technology Palampur for supporting the BIOLOG and FAME analysis and 16S rRNA gene sequencing of the isolates
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