Authors: Aarón MillánOropeza Luis FernándezLinares
Publish Date: 2016/06/06
Volume: 24, Issue: 33, Pages: 25618-25626
Abstract
The effect of sequential batch cultures of the marine microalgae Nannochloropsis oculata on lipid and biomass production was studied in 200L raceway ponds for 167 days nine harvesting cycles during winter and spring seasons under greenhouse conditions The highest biomass concentration and productivity were 12 g/L and 498 mg/L/day on days 73 5th cycle and 167 9th cycle respectively The overall interval of lipid production was between 131 and 530 mg/L Despite the daily and seasonal variations of light irradiance 0–1099 μmol photon/m2 s greenhouse temperature 21–507 °C and culture temperature 125–314 °C ANOVA analysis showed no statistical difference p value 001 on the fatty acid methyl ester FAMES composition over the nine harvesting cycles evaluated The most abundant FAMES were palmitic C160 stearic C180 and palmitoleic C161∆9 acids with 371 286 and 84 respectively The sequential batch cultures of N oculata in raceway ponds showed an increasing biomass production in each new cycle while keeping the quality of the fatty acid mixture under daily and seasonal variations of light irradiance and temperatureThe authors are grateful for the financial support provided by the National Science and Technology Council CONACYT of Mexico the Secretary for Research and Postgraduated Studies—Instituto Politécnico Nacional SIPIPN grants 20130388 and 20144620 and the collaboration of Research and Development Center of CARSO The authors would also like to thank Claudia Guerrero Barajas and Hervé Marie for their participation in reviewing the paper
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