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Title of Journal: Environ Sci Pollut Res

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Abbravation: Environmental Science and Pollution Research

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Springer Berlin Heidelberg

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1614-7499

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Combined use of PAH levels and EROD activities in

Authors: Azra Bozcaarmutlu Canan Sapmaz Gizem Kaleli Sema Turna Serpil YenisoyKarakaş
Publish Date: 2014/10/23
Volume: 22, Issue: 4, Pages: 2515-2525
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Abstract

The aim of this study was to determine the extent of polycyclic aromatic hydrocarbon PAH pollution by measuring PAH levels and 7ethoxyresorufinOdeethylase EROD activities in flathead mullet Mugil cephalus samples caught from the West Black Sea coast of Turkey The fish samples were caught in August 2008–2011 The levels of 13 PAHs were measured by highperformance liquid chromatography HPLC in the liver of fish Most of the measured PAHs had three rings low molecular weight The frequencies of detection of PAHs were higher in fish samples caught from Zonguldak Harbour and Gülüç Stream Mouth than those from Sakarya River Mouth Amasra and Kefken EROD activities and cytochrome P4501A CYP1A protein level were also measured in the fish liver microsomes Highly elevated EROD activities and CYP1A levels were measured in the mullet samples caught from Zonguldak Harbour and Gülüç Stream than those from Amasra and Kefken The detection of PAHs in the liver of fish samples shows recent exposure to PAHs The chemical analyses of PAHs and EROD activity results together reflected the extent of PAH pollution in the livers of fish caught from the West Black Sea coast of Turkey The results indicate that Zonguldak Harbour is the most polluted site in the West Black Sea coast of TurkeyThe authors thank all the members of the Republic of Turkey Ministry of Food Agriculture and Livestock Türkiye Cumhuriyeti Gıda Tarım ve Hayvancılık Bakanlığı for their help in collecting the fish They also would like to thank Ilker Kopru for his help in HPLC analysis and Prof Dr Aysel Kukner for her technical support in laboratory studies This research was supported by The Scientific and Technological Research Council of Turkey TUBITAK Project No 104Y083


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