Paper Search Console

Home Search Page About Contact

Journal Title

Title of Journal: J Assist Reprod Genet

Search In Journal Title:

Abbravation: Journal of Assisted Reproduction and Genetics

Search In Journal Abbravation:

Publisher

Springer US

Search In Publisher:

DOI

10.1007/s004240000305

Search In DOI:

ISSN

1573-7330

Search In ISSN:
Search In Title Of Papers:

The use of parthenotegenetic and IVF bovine blasto

Authors: R R Ruggeri Y Watanabe F Meirelles F F Bressan N Frantz A BosMikich
Publish Date: 2012/09/29
Volume: 29, Issue: 10, Pages: 1039-1043
PDF Link

Abstract

Clinical application of human embryonic stem cells will be possible when cell lines are created under xenofree and defined conditions We aimed to establish methodologies for parthenogenetic activation culture to blastocyst and mechanical isolation of the inner cell mass ICM using bovine oocytes as a model for derivation and proliferation of human embryonic stem cells under defined xenofree culture conditionsCumulusoocytecomplexes were in vitro matured and activated using Ca2+Ionophore and 6DMAP or in vitro fertilized IVF Parthenotes and biparental embryos were cultured to blastocysts when their ICM was mechanically isolated and placed onto a substrate of fibronectin in StemPro® medium After attachment primary colonies were left to proliferate and stained for pluripotency markers alkaline phosphatase and Oct4Parthenogenesis and fertilization presented significantly different success rates 91 and 79  respectively and blastocyst formation 40 and 43  respectively ICMs from parthenogenetic and IVF embryos formed primary and expanded colonies at similar rates 39  and 33  respectively Six out of eight parthenogenetic colonies tested positive for alkaline phosphatase Three colonies were analyzed for Oct4 and they all tested positive for this pluripotency markerOur data show that Ca2+ Ionophore and 6DMAP are efficient in creating large numbers of blastocysts to be employed as a model for human oocyte activation and embryo development After mechanical isolation parthenogetic derived ICMs showed a good rate of derivation in fibronectin and StemPro forming primary and expanded colonies of putative embryonic stem cells This methodology may be a good strategy for parthenogenetic activation of discarded human oocytes and derivation in defined conditions for future therapeutic interventions


Keywords:

References


.
Search In Abstract Of Papers:
Other Papers In This Journal:

  1. Time-lapse evaluation of human embryo development in single versus sequential culture media—a sibling oocyte study
  2. Clinical evaluation of two formulations of slow-freezing solutions for cleavage stage embryos
  3. Apoptotic sperm biomarkers and their correlation with conventional sperm parameters and male fertility potential
  4. Dehydroepiandrosterone (DHEA) supplementation results in supraphysiologic DHEA-S serum levels and progesterone assay interference that may impact clinical management in IVF
  5. CYP2C19 polymorphism increases the risk of endometriosis
  6. CYP2C19 polymorphism increases the risk of endometriosis
  7. Nuclei size in relation to nuclear status and aneuploidy rate for 13 chromosomes in donated four cells embryos
  8. Fifteen year follow-up of embryos cryopreserved in cancer patients for fertility preservation
  9. Live birth following transfer of a cryopreserved embryo generated from a cryopreserved oocyte and a cryopreserved sperm: Case report
  10. Use of the natural cycle and vitrification thawed blastocyst transfer results in better in-vitro fertilization outcomes
  11. Chromosome distribution of early miscarriages with present or absent embryos: female predominance
  12. Derivation of human embryonic stem cell lines from single blastomeres of low-quality embryos by direct plating
  13. Evaluation of in vitro fertilization parameters and estrogen receptor alpha gene polymorphisms for women with unexplained infertility
  14. Effect of the size of zona pellucida opening by laser assisted hatching on clinical outcome of frozen cleaved embryos that were cultured to blastocyst after thawing in women with multiple implantation failures of embryo transfer: a retrospective study
  15. IVF results in patients with very low serum AMH are significantly affected by chronological age
  16. Serum estradiol:oocyte ratio as a predictor of reproductive outcome: an analysis of data from >9000 IVF cycles in the Republic of Ireland
  17. Abnormally low expression of connexin 37 and connexin 43 in subcutaneously transplanted cryopreserved mouse ovarian tissue
  18. Association of WNT4 polymorphisms with endometriosis in infertile patients
  19. Day 3 ET, single blastocyst transfer (SBT) or frozen-thawed embryo transfer (FET): which is preferable for high responder patients in IVF/ICSI cycles?
  20. Sequential (hFSH + recFSH) vs homogenous (hFSH or recFSH alone) stimulation: clinical and biochemical (cumulus cell gene expression) aspects
  21. Correlation between follicular diameters and flushing versus no flushing on oocyte maturity, fertilization rate and embryo quality
  22. The role of epigenetics in idiopathic male infertility
  23. Deliveries of babies with normal health derived from oocytes with smooth endoplasmic reticulum clusters
  24. Dicer is a key player in oocyte maturation
  25. The vascular endothelial growth factor ( VEGF ) +405 G/C polymorphism and its relationship with recurrent implantation failure in women in an IVF programme with ICSI
  26. Clinically applied procedures for human ovarian tissue cryopreservation result in different levels of efficacy and efficiency
  27. Comparison of differences in development potentials between frozen-thawed D5 and D6 blastocysts and their relationship with pregnancy outcomes
  28. Luteal phase GnRHa trigger in random start fertility preservation cycles
  29. Feasibility and results of a modified intrafollicular insemination technique for treating primary infertility
  30. Caspase-3 and VEGF immunopositivity in seminiferous tubule germ cells in cases of obstructive and non-obstructive azoospermia in smokers versus non-smokers
  31. Relative expression of genes encoding SMAD signal transduction factors in human granulosa cells is correlated with oocyte quality
  32. Single nucleotide polymorphisms in the Anti-Müllerian hormone (AMH Ile 49 Ser) and Anti-Müllerian hormone type II receptor (AMHRII −482 A>G) as genetic markers in assisted reproduction technology
  33. Single thawed euploid embryo transfer improves IVF pregnancy, miscarriage, and multiple gestation outcomes and has similar implantation rates as egg donation
  34. Using the oocyte donation model to identify early trophoblast pregnenolone production
  35. Uterine vascularization and pregnancy outcome in patients undergoing intracytoplasmatic sperm injection: The role of nitric oxide
  36. Lack of carbon air filtration impacts early embryo development
  37. The importance of the cleavage stage morphology evaluation for blastocyst transfer in patients with good prognosis
  38. Improving fertilization rate in ICSI cycles by adding myoinositol to the semen preparation procedures: a prospective, bicentric, randomized trial on sibling oocytes
  39. Matrix metalloproteinases in serum and the follicular fluid of women treated by in vitro fertilization
  40. Bu Shen Tiao Chong recipe restores diminished ovary reserve through the BDNF pathway
  41. Predictors of treatment failure in young patients undergoing in vitro fertilization

Search Result: