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Title of Journal: J Assist Reprod Genet

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Abbravation: Journal of Assisted Reproduction and Genetics

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Springer US

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DOI

10.1002/chin.201131034

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ISSN

1573-7330

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Derivation of human embryonic stem cell lines from

Authors: Gang Yang Qingyun Mai Tao Li Canquan Zhou
Publish Date: 2013/07/09
Volume: 30, Issue: 7, Pages: 953-961
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Abstract

Blastomeres were isolated from normally fertilized day3 preimplantation LQ embryos by dissolving of the zona pellucida and were then plated directly onto inactivated human foreskin fibroblasts The subsequent culture was identical to that used to derive a hESC line from the inner cell mass of a blastocyst The established hESC lines were passaged and characterizedTwo hESC lines were produced by culturing the blastomeres individually in a hESC culture system hESCCS Both of the hESC lines maintained a normal 46chromosome XY karyotype expressed stemness markers and showed a pluripotent phenotype including the ability to differentiate into all three germ layers in vitro and in vivo

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Other Papers In This Journal:
  1. Time-lapse evaluation of human embryo development in single versus sequential culture media—a sibling oocyte study
  2. Clinical evaluation of two formulations of slow-freezing solutions for cleavage stage embryos
  3. Apoptotic sperm biomarkers and their correlation with conventional sperm parameters and male fertility potential
  4. Dehydroepiandrosterone (DHEA) supplementation results in supraphysiologic DHEA-S serum levels and progesterone assay interference that may impact clinical management in IVF
  5. CYP2C19 polymorphism increases the risk of endometriosis
  6. CYP2C19 polymorphism increases the risk of endometriosis
  7. Nuclei size in relation to nuclear status and aneuploidy rate for 13 chromosomes in donated four cells embryos
  8. The use of parthenotegenetic and IVF bovine blastocysts as a model for the creation of human embryonic stem cells under defined conditions
  9. Fifteen year follow-up of embryos cryopreserved in cancer patients for fertility preservation
  10. Live birth following transfer of a cryopreserved embryo generated from a cryopreserved oocyte and a cryopreserved sperm: Case report
  11. Use of the natural cycle and vitrification thawed blastocyst transfer results in better in-vitro fertilization outcomes
  12. Chromosome distribution of early miscarriages with present or absent embryos: female predominance
  13. Evaluation of in vitro fertilization parameters and estrogen receptor alpha gene polymorphisms for women with unexplained infertility
  14. Effect of the size of zona pellucida opening by laser assisted hatching on clinical outcome of frozen cleaved embryos that were cultured to blastocyst after thawing in women with multiple implantation failures of embryo transfer: a retrospective study
  15. IVF results in patients with very low serum AMH are significantly affected by chronological age
  16. Serum estradiol:oocyte ratio as a predictor of reproductive outcome: an analysis of data from >9000 IVF cycles in the Republic of Ireland
  17. Abnormally low expression of connexin 37 and connexin 43 in subcutaneously transplanted cryopreserved mouse ovarian tissue
  18. Association of WNT4 polymorphisms with endometriosis in infertile patients
  19. Day 3 ET, single blastocyst transfer (SBT) or frozen-thawed embryo transfer (FET): which is preferable for high responder patients in IVF/ICSI cycles?
  20. Sequential (hFSH + recFSH) vs homogenous (hFSH or recFSH alone) stimulation: clinical and biochemical (cumulus cell gene expression) aspects
  21. Correlation between follicular diameters and flushing versus no flushing on oocyte maturity, fertilization rate and embryo quality
  22. The role of epigenetics in idiopathic male infertility
  23. Deliveries of babies with normal health derived from oocytes with smooth endoplasmic reticulum clusters
  24. Dicer is a key player in oocyte maturation
  25. The vascular endothelial growth factor ( VEGF ) +405 G/C polymorphism and its relationship with recurrent implantation failure in women in an IVF programme with ICSI
  26. Clinically applied procedures for human ovarian tissue cryopreservation result in different levels of efficacy and efficiency
  27. Comparison of differences in development potentials between frozen-thawed D5 and D6 blastocysts and their relationship with pregnancy outcomes
  28. Luteal phase GnRHa trigger in random start fertility preservation cycles
  29. Feasibility and results of a modified intrafollicular insemination technique for treating primary infertility
  30. Caspase-3 and VEGF immunopositivity in seminiferous tubule germ cells in cases of obstructive and non-obstructive azoospermia in smokers versus non-smokers
  31. Relative expression of genes encoding SMAD signal transduction factors in human granulosa cells is correlated with oocyte quality
  32. Single nucleotide polymorphisms in the Anti-Müllerian hormone (AMH Ile 49 Ser) and Anti-Müllerian hormone type II receptor (AMHRII −482 A>G) as genetic markers in assisted reproduction technology
  33. Single thawed euploid embryo transfer improves IVF pregnancy, miscarriage, and multiple gestation outcomes and has similar implantation rates as egg donation
  34. Using the oocyte donation model to identify early trophoblast pregnenolone production
  35. Uterine vascularization and pregnancy outcome in patients undergoing intracytoplasmatic sperm injection: The role of nitric oxide
  36. Lack of carbon air filtration impacts early embryo development
  37. The importance of the cleavage stage morphology evaluation for blastocyst transfer in patients with good prognosis
  38. Improving fertilization rate in ICSI cycles by adding myoinositol to the semen preparation procedures: a prospective, bicentric, randomized trial on sibling oocytes
  39. Matrix metalloproteinases in serum and the follicular fluid of women treated by in vitro fertilization
  40. Bu Shen Tiao Chong recipe restores diminished ovary reserve through the BDNF pathway
  41. Predictors of treatment failure in young patients undergoing in vitro fertilization
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