Authors: Erika M Munch Amy E Sparks Hakan E Duran Bradley J Van Voorhis
Publish Date: 2015/05/24
Volume: 32, Issue: 7, Pages: 1009-1017
Abstract
This is a retrospective cohort analysis of fresh controlled ovarian hyperstimulation cycles as well as previously cryopreserved pronuclear stage embryo transfer cycles in a single IVF center Embryo development and cyclebased outcomes were compared among three groups 1 when carbon filtration was present 2 when carbon filtration was absent and 3 when carbon filtration had been restoredA total of 524 fresh cycles and 156 cryopreserved embryo cycles were analyzed Fertilization cleavage and blastocyst conversion rates for fresh cycles all declined during the period of absent carbon filtration and recovered after the restoration of carbon filtration Cryopreserved embryos that were thawed and cultured during the period of absent filtration did not have changes in cleavage or blastocyst conversion rates compared to periods where carbon filtration was present Clinical pregnancy and live birth rates were unchanged among the three time periodsThe absence of carbon filtration in an IVF laboratory air handler is associated with poor fertilization and early embryo development for fresh cycles Because development of previously frozen pronuclear stage embryos was unaffected the lack of carbon filtration may preferentially affect embryos in the perifertilization period Carbon filtration is an integral part to a successful human invitro fertilization laboratory
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