Authors: Harsh Raman Rosy Raman Rachel Wood Peter Martin
Publish Date: 2006/08/17
Volume: 18, Issue: 2, Pages: 171-183
Abstract
ALMT1 gene encoding a membrane protein that facilitates an aluminium stimulated malate efflux has been characterised and mapped in wheat Triticum aestivum L Here we have identified molecular markers targeting insertion/deletion indel and SSR repeats within intron 3 region of the ALMT1 gene Both the markers ALMT1SSR3a and ALMT1SSR3b based on repetitive indels exhibited complete cosegregation with Al tolerance malate efflux and a CAPS marker discriminating ALMT11 and ALMT12 alleles in a doubled haploid population derived from Diamondbird Altolerant/Janz Alsensitive A parental screen of 20 diverse wheat genotypes with repetitive indel markers indicated that six allele variants exist at the ALMT1SSR3 locus Sequence analysis confirmed that these variations were due to indels copy number of SSR repeats and base substitution within SSR repeats The higher level of variation in intron 3 suggests that this genomic region has been constrained by indels SSR and single nucleotide polymorphisms Results have proven that repetitive indel markers cosegregating with the Al tolerance locus will be useful for marker assisted selection and population and evolution studiesWe greatly acknowledge the NSW Agricultural Genomics Centre of BioFirst Initiative of NSW Government and NSWDPI for supporting this project We thank Fiona Wray for technical assistance and Greg Grimes Australian Winter Cereal Collection Tamworth for providing seeds of some wheat accessions
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