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Title of Journal: Mol Breeding

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Abbravation: Molecular Breeding

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Springer Netherlands

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DOI

10.1016/j.actamat.2009.11.004

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ISSN

1572-9788

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In silico mapping of 1758 new SSR markers develope

Authors: Manli Li Nana Yuyama Le Luo Mariko Hirata Hongwei Cai
Publish Date: 2009/03/01
Volume: 24, Issue: 1, Pages: 41-47
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Abstract

In order to develop simple sequence repeat SSR markers for sorghum a total of 2113 primer pairs were designed from 81342 public genomic sequence contigs of sorghum methylfiltered sequences downloaded from SAMI Sorghum Assembled Genomic Island After screening eight sorghum lines 1758 primers amplifying polymorphisms were identified Of these 1710 SSR markers were predominantly polymorphic in the eight lines and could detect from two to eight alleles In addition 1692 of the markers were mapped on to the ten sorghum chromosomes by in silico mapping The number of markers per chromosome ranged from 107 to 285 These SSR markers will provide an ideal marker system to help with gene targeting gene cloning quantitative trait locus mapping and markerassisted selection in sorghum speciesThis study was supported financially by a grant from the Japan Racing Association Ms M Li is grateful to the China Scholarship Council Grant No 2007 3020 for supporting her stay at the Forage Crop Research Institute of the Japan Grassland Agriculture and Forage Seed Association We also thank Dr Alan V Stewart PGG Wrightson Seeds Lincoln New Zealand and the two anonymous reviewers for their valuable comments on this manuscript

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