Authors: Yuqing Lu Xiaoyang Wu Miaomiao Yao Jinpeng Zhang Weihua Liu Xinming Yang Xiuquan Li Juan Du Ainong Gao Lihui Li
Publish Date: 2015/03/13
Volume: 35, Issue: 3, Pages: 96-
Abstract
The wheat relative Agropyron cristatum L Gaertn 2n = 4x = 28 genomes PPPP has often been used as a donor of useful genetic variation for wheat improvement including enhanced disease resistance to powdery mildew caused by Blumeria graminis f sp tritici Bgt In this report resistance to powdery mildew was transferred from A cristatum to common wheat and the resulting introgression line PB3558 exhibited allstage resistance To identify the resistance gene genetic analysis was conducted using F2 F23 and recombinant inbreed line populations derived from the cross of PB3558 and the susceptible cultivar Jing 4841 Segregation ratios from inoculation with Bgt isolate E09 indicated that the resistance was conferred by a single dominant gene temporarily designated PmPB3558 Bulked segregant analysis BSA was applied to screen for molecular markers linked to PmPB3558 and five published markers were found In order to increase the density of the genetic map we developed ten novel single sequence repeat markers based on the single nucleotide polymorphism SNP loci with polymorphisms produced from a combination wheat 90 k SNP array and BSA PmPB3558 was located on wheat chromosome arm 5DS and flanked by markers Xcfd81 and Xbwm25 Because there are other powdery mildew resistance genes located on 5DS 21 Bgt isolates were used to compare the reaction differences PmPB3558 showed unique reactions suggesting that it was most likely a novel allele This is the first documentation on transferring an alien powdery mildew resistance gene from A cristatum and the germplasm acquired in this study will be useful for broadening the genetic basis for wheat breedingThis work was funded by the National Natural Science Foundation of China No 31271714 and the National Basic Research Program of China the 973 program No 2011CB100104 We would like to thank Dr Hongjie Li at the Institute of Crop Sciences Chinese Academy of Agricultural Sciences for help with the assessment of Bgt isolates
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