Authors: Zhiyuan Fu Zhan Zhao Yongtian Qin Mengmeng Xu Yongqiang Chen Jihua Tang
Publish Date: 2016/11/21
Volume: 36, Issue: 12, Pages: 159-
Abstract
Germination and synchronous seedling emergence are necessary for crop yield One differentially expressed Cupin protein during maize seed germination had been identified in our previous study To elucidate the functional sites in its coding gene ZmGLP a diverse maize association population was assayed In alignment with B73 the ZmGLP gene contains five exons and encodes 522 amino acids and a potential signal peptide domain from the 7 to 22 amino acids and a typical Cupin domain from the 332 to 477 amino acids were identified The ZmGLP has a quick linkage disequilibrium decay with about 300 bp physical distance in the analyzed sequences which implied that human selection might be undone in this gene High genetic variations were evidenced in ZmGLP gene with 118 polymorphic sites in the association population An Indel9 18 bp in the fifth exon of the intermotif region of the second Cupin domain which influences the formation of C coil in the barrel folding was detected significantly associated with germination vigor of maize seeds The 18bp insertion which might help keeping the barrel folding intact was a favorable allele for relative high germination vigor Evolution analysis showed that this 18bp deletion was a lossoffunction mutation However the action mode of ZmGLP is still yet to be further studied for maize improvementThis research was supported by the National Natural Science Foundation of China 31000715 the State Key Laboratory of Wheat and Maize Crop Science SKL2014ZH09 and the Shanghai Science and Technology Committee 11DZ2272100 The funders had no role in study design data collection and analysis decision to publish or preparation of the manuscript
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