Authors: Xinyi Wu Ruiru Cheng Shulin Xue Zhongxin Kong Hongshen Wan Guoqiang Li Yulong Huang Haiyan Jia Jizeng Jia Lixia Zhang Zhengqiang Ma
Publish Date: 2013/08/21
Volume: 33, Issue: 1, Pages: 129-138
Abstract
The spike characteristics length spikelet density and fertile floret number are related yield components and are important in cereal improvement QSplnau2D is a major quantitative trait locus controlling spike length SPL detected in the recombinant inbred line population developed by crossing wheat Triticum aestivum cultivars Nanda2419 with Wangshuibai In this study to validate its genetic effect and determine its precise location QSplnau2D’s nearisogenic line NIL was developed using Mianyang99323 as the recurrent parent through markerassisted selection Field trials showed that the NIL not only had significantly longer spikes on average than the recurrent parent but also had significantly higher grain weight but did not differ in spikelet number and kernel number per spike In the F2 population derived from a cross of the NIL with Mianyang99323 QSplnau2D functioned like a single gene and conditioned the SPL in a partially dominant manner and was thus designated as HL1 for head length To precisely map HL1 89 recombinants consisting of 11 genotypes were identified in the NILderived F2 population of 674 plants by using markers in the Xwmc25–Xgpw4080 interval Phenotyping these lines showed that the introduction of a 09cM interval flanked by Xcfd53 and DG371 in Nanda2419 resulted in longer spikes and a higher grain weight in the NIL The availability of markers closely linked to HL1 could facilitate its use in breeding programsWe are grateful to Mingcheng Luo at University of California Davis for providing SNP sequences This study was partially supported by ‘973’ program 2011CB100103 ‘863’ program 2012AA101105 NSFC programs 30025030 30721140555 Seed Funds for the Central Universities 2012 ‘111’ project B08025 and PAPD project of Jiangsu Higher Education Institutions
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