Authors: Kyle Rak Paul C Bethke Jiwan P Palta
Publish Date: 2017/01/24
Volume: 37, Issue: 2, Pages: 15-
Abstract
Cultivated potato Solanum tuberosum L is a highly heterozygous autotetraploid crop species and this creates challenges for traditional line development and molecular breeding Recent availability of a singlenucleotide polymorphism SNP array with 8303 features and software packages for linkage and association mapping in autotetraploid species present new opportunities for the identification of genomic regions that contribute to highvalue traits in cultivated potato A biparental tetraploid potato population was evaluated across three field seasons and storage trials in order to identify quantitative trait loci QTL for multiple tuber traits including fried chip color after 55–72 °C storage Tetraallelic dosage information was used to construct a genetic linkage map that covered 1041 cM and contained 2095 SNP markers with a median marker interval of 04 cM A total of 41 QTL were identified for flower color tuber yield tuber number per plant tuber weight tuber size and chip color after various storage regimes Moderate effect QTL for chip color at 3 months were identified that colocalized with candidate genes vacuolar invertase VInv invertase inhibitor INH2 and apoplastic invertase Inv ap b A separate QTL for chip color after 6 months of storage was identified in the short arm of chromosome 2 and this locus may contribute to variation in senescent sweetening resistance QTL for tuber weight length and width colocalized with a known QTL for plant maturity on chromosome 5 Genomewide association mapping using a polyploid model detected the tuber size QTL and identified a number of candidate SNPs but was unable to detect markers significantly associated with chip color
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