Inheritance of seed coat color genes in Emphasis

Authors: Mukhlesur Rahman Genyi Li Dana Schroeder Peter B E McVetty

Publish Date: 2010/01/20

Volume: 26, Issue: 3, Pages: 439-453

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Abstract

Seed coat color inheritance in Brassica napus was studied in F1 F2 F3 and backcross progenies from crosses of five black seeded varieties/lines to three pure breeding yellow seeded lines Maternal inheritance was observed for seed coat color in B napus but a pollen effect was also found when yellow seeded lines were used as the female parent Seed coat color segregated from black to dark brown light brown dark yellow light yellow and yellow Seed coat color was found to be controlled by three genes the first two genes were responsible for black/brown seed coat color and the third gene was responsible for dark/light yellow seed coat color in B napus All three seed coat color alleles were dominant over yellow color alleles at all three loci Sequence related amplified polymorphism SRAP was used for the development of molecular markers cosegregating with the seed coat color genes A SRAP marker SA12BG18388 tightly linked to one of the black/brown seed coat color genes was identified in the F2 and backcross populations This marker was found to be anchored on linkage group A9/N9 of the Agenome of B napus This SRAP marker was converted into sequencecharacterized amplification region SCAR markers using chromosomewalking technology A second SRAP marker SA7BG29245 very close to another black/brown seed coat color gene was identified from a high density genetic map developed in our laboratory using primer walking from an anchoring marker The marker was located on linkage group C3/N13 of the Cgenome of B napus This marker also cosegregated with the black/brown seed coat color gene in B rapa Based on the sequence information of the flanking sequences 24 single nucleotide polymorphisms SNPs were identified between the yellow seeded and black/brown seeded lines SNP detection and genotyping clearly differentiated the black/brown seeded plants from dark/light/yellowseeded plants and also differentiated between homozygous Y2Y2 and heterozygous Y2y2 black/brown seeded plants A total of 768 SRAP primer pair combinations were screened in dark/light yellow seed coat color plants and a close marker DC1GA27197 linked to the dark/light yellow seed coat color gene was developed These three markers linked to the three different yellow seed coat color genes in B napus can be used to screen for yellow seeded lines in canola/rapeseed breeding programsThe authors gratefully acknowledge the financial support for this research provided by NSERC Bunge Canada the Manitoba Canola Growers Association and the University of Manitoba The provision of the three yellow seeded lines by Norddeutsche Pflanzenzucht NPZ Lembke Hohenlieth Germany is gratefully acknowledged M Rahman gratefully acknowledges NSERC the Faculty of Graduate Studies University of Manitoba and the Government of Manitoba for their scholarship support M Rahman also wishes to thank the University of Manitoba Students’ Union the Douglas L Campbell Graduate Fellowship committee and the Clarence Bogardus Sharpe Memorial Scholarship committee for their financial support

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