Authors: Stefano Pavan Adalgisa Schiavulli Michela Appiano Cecilia Miacola Richard G F Visser Yuling Bai Concetta Lotti Luigi Ricciardi
Publish Date: 2012/09/08
Volume: 31, Issue: 1, Pages: 247-253
Abstract
Powdery mildew is the most widespread disease of pea Pisum sativum L and causes severe economic losses worldwide Recessively inherited er1 powdery mildew resistance successfully used for decades in pea breeding programs has recently been shown to originate from the loss of function of the PsMLO1 gene Five er1 alleles each corresponding to a different PsMLO1 null mutation have been characterized to date in pea germplasm In order to aid er1 selection we aimed to identify functional markers which target PsMLO1 polymorphisms directly responsible for the resistant phenotype Highly informative cleaved amplified polymorphic sequence CAPS derived cleaved amplified polymorphic sequence dCAPS sequence tagged site STS and highresolution melting HRM markers were developed which enable the selection of each of the five er1 alleles Taken together the results described here provide a powerful tool for breeders overcoming limitations of previously reported er1linked markers due to the occurrence of recombination with the resistance locus and/or the lack of polymorphism between parental genotypes The HRM marker er15/HRM54 reported here targeting a mutagenesisinduced er1 allele recently described by us does not require manual processing after PCR amplification and is therefore suitable for largescale breeding programs based on highthroughput automated screening
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