Paper Search Console

Home Search Page About Contact

Journal Title

Title of Journal: Mol Breeding

Search In Journal Title:

Abbravation: Molecular Breeding

Search In Journal Abbravation:

Publisher

Springer Netherlands

Search In Publisher:

DOI

10.1002/pssb.19700400105

Search In DOI:

ISSN

1572-9788

Search In ISSN:
Search In Title Of Papers:

Ectopic overexpression of a mungbean vacuolar NaS

Authors: Sagarika Mishra Ratikanta Behura Jay Prakash Awasthi Mohitosh Dey Debeeprasad Sahoo Sudipta Shekhar Das Bhowmik Sanjib Kumar Panda Lingaraj Sahoo
Publish Date: 2014/07/30
Volume: 34, Issue: 3, Pages: 1345-1359
PDF Link

Abstract

Salinity is a major threat to sustainable agriculture worldwide Plant NHX exchangers play an important role in conferring salt tolerance under salinity stress In this study a vacuolar Na+/H+ antiporter gene VrNHX1 Genbank Accession No JN6562111 from mungbean Vigna radiata was introduced into cowpea Vigna unguiculata by the Agrobacterium tumefaciensmediated transformation method Polymerase chain reaction and Southern blot hybridization confirmed the stable integration of VrNHX1 into the cowpea genome Comparative expression analysis by semiquantitative RTPCR revealed higher expression of VrNHX1 in transgenic cowpea plants than wildtype Under salt stress conditions T2 transgenic 35SVrNHX1 cowpea lines exhibited higher tolerance to 200 mM NaCl treatment than wildtype Furthermore T2 transgenic 35SVrNHX1 lines maintained a higher K+/Na+ ratio in the aerial parts under salt stress and accumulated higher Na+ in roots than wildtype Physiological analysis revealed lower levels of lipid peroxidation hydrogen peroxide and oxygen radical production but higher levels of relative water content and proline ascorbate and chlorophyll contents in T2 transgenic 35SVrNHX1 linesWe express our sincere thanks to Prof Sampa Das Bose Institute for providing the Agrobacterium tumefaciens strain EHA105 and the Center for Application of Molecular Biology to International Agriculture CAMBIA Australia for pCAMBIA2301 The research was supported by Grants from the Department of Biotechnology Government of India BT/PR10818/AGR/02/591/2008 and BT/01/NE/PS/08 SM is grateful to MHRD for Research Fellowship

Keywords:

References

.
Search In Abstract Of Papers:
Other Papers In This Journal:
  1. Improvement of the recessive genic male sterile lines with a subgenomic background in Brassica napus by molecular marker-assisted selection
  2. Genetically engineered anthocyanin pathway for high health-promoting pigment production in eggplant
  3. High-resolution mapping and gene prediction of Xanthomonas Oryzae pv. Oryzae resistance gene Xa7
  4. Chromosome engineering in wheat to restore male fertility in the msH1 CMS system
  5. Marker imputation efficiency for genotyping-by-sequencing data in rice ( Oryza sativa ) and alfalfa ( Medicago sativa )
  6. In silico mapping of 1758 new SSR markers developed from public genomic sequences for sorghum
  7. Chromosome segregation in an allotetraploid banana hybrid (AAAB) suggests a translocation between the A and B genomes and results in eBSV-free offsprings
  8. Analysis of gene-derived SNP marker polymorphism in US wheat ( Triticum aestivum L.) cultivars
  9. Diversity arrays technology (DArT) markers in apple for genetic linkage maps
  10. Single nucleotide polymorphism in wheat chromosome region harboring Fhb1 for Fusarium head blight resistance
  11. Association mapping of stigma and spikelet characteristics in rice ( Oryza sativa L.)
  12. Marker-assisted breeding of Thai fragrance rice for semi-dwarf phenotype, submergence tolerance and disease resistance to rice blast and bacterial blight
  13. Genetic mapping of a putative Agropyron cristatum -derived powdery mildew resistance gene by a combination of bulked segregant analysis and single nucleotide polymorphism array
  14. Repetitive Indel Markers within the ALMT1 Gene Conditioning Aluminium Tolerance in Wheat ( Triticum aestivum L.)
  15. High frequency of chromosome deletions in regenerated and mutagenized apple ( Malus ×  domestica Borkh.) seedlings
  16. Identification of glutinous maize landraces and inbred lines with altered transcription of waxy gene
  17. Fine mapping and identification of candidate Br - or gene controlling orange head of Chinese cabbage ( Brassica rapa L. ssp. pekinensis )
  18. Inheritance of seed coat color genes in Brassica napus (L.) and tagging the genes using SRAP, SCAR and SNP molecular markers
  19. A genotyping platform assembled with high-throughput DNA extraction, codominant functional markers, and automated CE system to accelerate marker-assisted improvement of rice
  20. Genome-wide association mapping for grain yield components and root traits in Brassica juncea (L.) Czern & Coss.
  21. Rapid method for detecting SNPs on agarose gels and its application in candidate gene mapping
  22. TILLING by Sequencing (TbyS) for targeted genome mutagenesis in crops
  23. A plant-transformation-competent BIBAC library of ginseng ( Panax ginseng C. A. Meyer) for functional genomics research and characterization of genes involved in ginsenoside biosynthesis
  24. Molecular mapping of powdery mildew resistance gene PmHNK in winter wheat ( Triticum aestivum L.) cultivar Zhoumai 22
  25. Enhanced tolerance of transgenic sweetpotato plants that express both CuZnSOD and APX in chloroplasts to methyl viologen-mediated oxidative stress and chilling
  26. Validation of DGAT1 - 2 polymorphisms associated with oil content and development of functional markers for molecular breeding of high-oil maize
  27. Fine mapping and molecular marker development of anthocyanin absent , a seedling morphological marker for the selection of male sterile 10 in tomato
  28. Transcriptome sequencing and de novo analysis of a recessive genic male sterile line in cabbage ( Brassica oleracea L. var. capitata)
  29. Quantitative trait loci for agronomic traits in an elite barley population for Mediterranean conditions
  30. Molecular characterization and detection of a spontaneous mutation conferring imidazolinone resistance in rapeseed and its application in hybrid rapeseed production
  31. Application of SSR markers for molecular characterization of hybrid parents and purity assessment of ICPH 2438 hybrid of pigeonpea [ Cajanus cajan (L.) Millspaugh]
  32. QTL mapping of potato chip color and tuber traits within an autotetraploid family
  33. Marker-assisted selection provides arabica coffee with genes from other Coffea species targeting on multiple resistance to rust and coffee berry disease
  34. Functional marker related to germination vigor of maize seed
  35. Cereal flag leaf adaptations for grain yield under drought: knowledge status and gaps
  36. Precise mapping of a quantitative trait locus interval for spike length and grain weight in bread wheat ( Triticum aestivum L.)
  37. Loss of function of OsMADS34 leads to large sterile lemma and low grain yield in rice ( Oryza sativa L.)
  38. De novo transcriptome sequencing-based discovery and expression analyses of verbascoside biosynthesis-associated genes in Rehmannia glutinosa tuberous roots
  39. Superoxide dismutase multigene family in longan somatic embryos: a comparison of CuZn-SOD, Fe-SOD, and Mn-SOD gene structure, splicing, phylogeny, and expression
  40. Identification of a complete set of functional markers for the selection of er1 powdery mildew resistance in Pisum sativum L.
  41. Optimized breeding strategies for multiple trait integration: II. Process efficiency in event pyramiding and trait fixation
  42. QTL mapping for yield and photosynthetic related traits under different water regimes in wheat
  43. Tan spot susceptibility governed by the Tsn1 locus and race-nonspecific resistance quantitative trait loci in a population derived from the wheat lines Salamouni and Katepwa
Search Result: