Authors: S Salvi S Piazza S Predieri P Fuochi R Velasco M Malnoy
Publish Date: 2015/01/15
Volume: 35, Issue: 1, Pages: 4-
Abstract
Collections of deletion mutants are important tools for functional genomics in crops In order to establish an efficient physical γraybased mutagenesis protocol and estimate the frequency of induced chromosome deletion events in apple we mutagenized a total of 338 leaves of the apple cultivar ‘Golden Delicious’ with a range of irradiation doses from 0 to 30 Gy After in vitro culture and regeneration a total of 248 plantlets were genotyped by a custommade 384 SNPbased array suited to identify homozygous regions corresponding to chromosome deletions produced by the irradiation and/or the in vitro culture treatments With this approach we identified nine multiSNP chromosome deletions corresponding to 37 of all regenerated plantlets and ranging from one to 44 cM 01–202 Mb Chromosome deletions were also observed at mildly not significantly lower frequency in plantlets regenerated from nonirradiated leaves Our results confirm the possibility to generate apple plants with sizeable deletions of known dimension and location opening the way to creating a collection of deletions spanning the apple genome useful for functional genomics The high frequency of deletions apparently caused by somaclonal variation deserves further investigation
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