Authors: Amy N Bernardo Hongxiang Ma Dadong Zhang Guihua Bai
Publish Date: 2011/04/03
Volume: 29, Issue: 2, Pages: 477-488
Abstract
Fusarium head blight FHB is a destructive disease that reduces wheat grain yield and quality To date the quantitative trait locus on 3BS Fhb1 from Sumai 3 has shown the largest effect on FHB resistance Single nucleotide polymorphism SNP is the most common form of genetic variation and is suitable for highthroughput markerassisted selection MAS We analyzed SNPs derived from 23 wheat expressed sequence tags ESTs that previously mapped near Fhb1 on chromosome 3BS Using 71 Ning 7840/Clark BC7F7 recombinant inbred lines and the singlebase extension method we mapped seven SNP markers between Xgwm533 and Xgwm493 flanking markers for Fhb1 Five of the SNPs explained 45–54 of the phenotypic variation for FHB resistance Haplotype analysis of 63 wheat accessions from eight countries based on SNPs in EST sequences simple sequence repeats and sequence tagged sites in the Fhb1 region identified four major groups 1 USClark 2 Asian 3 USErnie and 4 Chinese Spring The Asian group consisted of Chinese and Japanese accessions that carry Fhb1 and could be differentiated from other groups by marker Xsnp3BS11 All Sumai 3related accessions formed a subgroup within the Asian group and could be sorted out by Xsnp3BS8 The SNP markers identified in this study should be useful for MAS of Fhb1 and fine mapping to facilitate cloning of the Fhb1 resistance geneThis is contribution no 11032J from the Kansas Agricultural Experiment Station Manhattan Kansas USA This project is partly funded by the US Wheat and Barley Scab Initiative and the National Research Initiative Competitive Grants CAP project 20116800230029 from the USDA National Institute of Food and Agriculture Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture
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