Authors: G B Noumbissié M Chabannes F Bakry S Ricci C Cardi JC Njembele D Yohoume K Tomekpe ML IskraCaruana A D’Hont FC Baurens
Publish Date: 2016/03/24
Volume: 36, Issue: 4, Pages: 38-
Abstract
Many banana cultivars including the Plantain type are triploid interspecific hybrids between Musa acuminata A genome and Musa balbisiana B genome M balbisiana contains endogeneous Banana streak virus sequences eBSVs that can in interspecific genome context spontaneously release infectious viral genomes We analyzed a triploid progeny of 184 individuals from a cross between a tetraploid AAAB breeding accession CRBP39 and the diploid AA accession Pahang with 38 SSR and eBSVspecific PCR markers The results showed that 1 most of the alleles are found/transmitted in the expected frequency to the progeny with only 10 biased 2 70 of the loci displayed a tetrasomic allele segregation and 3 interspecific intrachromosomal recombinations occurred for all the chromosome segments surveyed However half of the offspring obtained resulted from maternal unbalanced gametes transmission Analysis of gamete composition and marker association suggested the presence of a large translocation between A and B genome involving chromosome 1 and 3 The two infectious eBSVs present in the maternal parent CRBP39 are located on chromosome 1B and appeared in a higher proportion than expected in the progeny Interestingly we showed that both eBSVs were absent from 24 offspring that represent promising material for breedingAD FCB MLC MC and FB jointly designed the study GBN FCB CC and AD designed generated and analyzed SSR genotyping data GBN MC and MLC designed experiment with eBSV markers and analyzed data SR FB JCN DY and KT designed and performed the genetic cross and handled the progeny AD and FCB wrote the manuscript MC and MLC edited the manuscript All authors discussed the results and implications and commented on the manuscript
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