Authors: Liyuan Zhang Zejun Huang Xiaoxuan Wang Jianchang Gao Yanmei Guo Yongchen Du Hong Hu
Publish Date: 2016/07/19
Volume: 36, Issue: 8, Pages: 107-
Abstract
Seedling morphology plays an important role in plant breeding via markerassisted selection The tomato Solanum lycopersicum mutant anthocyanin absent aa presents a green hypocotyl during the seedling stage This trait has been utilized in markerassisted selection of male sterile 10 ms 10 at the seedling stage because their corresponding loci are closely linked on chromosome 2 However the genetic basis of aa has not been ascertained to date In the present study aa was finemapped to a 963kb region on chromosome 2 that contained 14 putative genes Thereinto Solyc02g081340 which encodes a putative glutathione Stransferase was thereby designated SlGSTAA Phylogenetic analysis suggested that SlGSTAA was closely related to PhAN9 which is required for anthocyanin sequestration in petunia Petunia hybrida Realtime quantitative reverse transcription PCR analysis showed that the expression of the SlGSTAA gene was barely detected in aa mutants Sequencing revealed that there was a 34kb deletion in the aa region where the SlGSTAA gene was apparently deleted A codominant insertion/deletion marker was developed in line with the deletion to differentiate homozygous wildtype heterozygous and homozygous aa mutant genotypes The findings of the present study may facilitate the functional analysis of the SlGSTAA gene and markerassisted selection of aa and ms 10 in tomatoWe thank the Tomato Genetics Resource Center Davis CA USA and Esther van der Knaap’s laboratory at The Ohio State University Wooster OH USA for providing seed stocks This research was supported by the Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences CAASASTIPIVFCAAS the China Agriculture Research System CARS25 and the Special Fund for AgroScientific Research in the Public Interest of China 201203004
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