Authors: Zhen Huang Lu Xiao Xiaoling Dun Shengqian Xia Bin Yi Jing Wen Jinxiong Shen Chaozhi Ma Jinxing Tu Jinling Meng Tingdong Fu
Publish Date: 2010/12/02
Volume: 29, Issue: 1, Pages: 181-187
Abstract
Both the pollination control system and genetic distance are major factors in the utilization of crop heterosis The recessive genic male sterile line RGMS 77365A Bnms3ms3ms4ms4 has been widely applied to hybrid seed production because it can generate a completely male sterile population by crossing with the 77365C temporary line Bnms3ms3rfrf In this study the sterile genes of 77365A were transferred to the new Brassica napus lines 7749 and 7750 with a high content of subgenomes by backcross breeding We used the amplified fragment length polymorphism AFLP technique combined with bulk segregant analysis BSA to identify markers linked to the BnMs4 gene Twelve AFLP markers linked to the BnMs4 gene were identified Of them SA06MG09 and P08MG16 were the closest makers which were on either side of the gene at a distance of 09 and 08 cM respectively Twenty AFLP primer combinations were used to screen the F2 BC1F3 and BC2F4 populations from the breeding program and the markers linked to the BnMs3 and BnMs4 genes were used to screen the BC2F4 populations As a result we obtained two types of improved sterile lines 7749A and 7750A and their indexes of subgenomic components ISG were 442–498 and 202–266 respectively The combining ability analyses of seed yield character were conducted in the crosses from the three sterile lines and ten restorers within a random block design in three environments for two successive years The general combining ability GCA of the two improved sterile lines were significantly higher than the GCA of 77365A in every environment tested The two improved sterile lines had stability in seed yield and they will be used in the future for hybrid seed production
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