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Title of Journal: Mol Breeding

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Abbravation: Molecular Breeding

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Springer Netherlands

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DOI

10.1002/chin.201401154

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ISSN

1572-9788

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A genotyping platform assembled with highthroughp

Authors: Likai Chen Weiwei Gao Tao Guo Cuihong Huang Ming Huang Jiafeng Wang Wuming Xiao Guili Yang Yongzhu Liu Hui Wang Zhiqiang Chen
Publish Date: 2016/08/24
Volume: 36, Issue: 9, Pages: 123-
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Abstract

The introgression of multiple genes into traditional cultivars using markerassisted selection MAS in order to obtain favorable traits is an effective strategy to achieve improved rice lines Genotyping of markers is a central component of the evaluation of germplasm and the selection of progeny lines However efficient DNA extraction and genotyping of large breeding populations still remain limiting factors in rice molecular breeding programs This study has developed and validated a costeffective rapid 1 h for 96 samples and highthroughput 96well format total DNAextraction method based on magnetic particle technology To improve the grainquality traits of two rice varieties we have designed and employed an efficient codominant functional marker system including Wx ALK Chalk5 and fgr genes in combination with genotyping based on automated capillary electrophoresis Rice lines with simultaneous improvement at multiple loci were obtained and found to have superior grain quality and to be fragrant The genotyping pipeline established in this study represents an efficient reliable and precise platform for MASFinancial support for this research was provided in part by a grant from Science and Technology Planning Project of Guangdong Province China 2015B020231011 National Key Technology Research and Development Program of China No 2016YFD0102102 and the earmarked fund for Modern Agroindustry Technology Research System CARS0112

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