Authors: Junfeng Zhai Yi Wang Chunyu Sun Shicui Jiang Kangyu Wang Yang Zhang HongBin Zhang Meiping Zhang
Publish Date: 2012/12/22
Volume: 31, Issue: 3, Pages: 685-692
Abstract
Ginseng Panax ginseng C A Mey is widely used as a major medicinal herb and as a feedstock for the medicine beverage food cosmetic etc industries in China and several other Asian countries However limited research has been accomplished into its genetics genomics and breeding To clone characterize and utilize the genes of economic importance in the species we have developed a largeinsert planttransformationcompetent binary bacterial artificial chromosome BIBAC library for Jilin ginseng cv Damaya The library contains 141312 clones with an average insert size of 110 kb each likely containing approximately 20–30 genes The clones of the library have all been arrayed in 384well microplates and permanently archived We screened the library and identified BIBAC clones containing nine genes likely involved in the biosynthesis pathway of ginsenosides—the major medicinally effective compounds of ginseng—with approximately four BIBACs per gene This result further verified the quality of the library and demonstrated its utility in cloning characterization and utilization of economically important genes in ginseng Furthermore since the library is cloned in a planttransformationcompetent BIBAC vector pCLD04541 that can be directly transformed in a variety of plants via both the Agrobacteriummediated method and the particle bombardment method we have also demonstrated the stability of largeinsert ginseng DNA BIBACs in different Agrobacterium strains which is crucial to largeinsert BIBAC transformation in plants Therefore the Jilin ginseng BIBAC library provides resources and tools useful for functional genomics research and cloning characterization and utilization of economically important genes in the species
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