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Title of Journal: Mol Breeding

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Abbravation: Molecular Breeding

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Springer Netherlands

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DOI

10.1007/s10847-010-9900-7

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ISSN

1572-9788

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Application of SSR markers for molecular character

Authors: Rachit K Saxena Kulbhushan Saxena Rajeev K Varshney
Publish Date: 2010/05/26
Volume: 26, Issue: 2, Pages: 371-380
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Abstract

With an objective of achieving a breakthrough in the productivity of pigeonpea a hybrid breeding technology based on elements of the cytoplasmicnuclear malesterility CMS system and partial natural outcrossing has recently been developed However there is no molecular diversity information available on parental lines of hybrids being generated at the International Crops Research Institute for the SemiArid Tropics ICRISAT This study deals with the use of 148 simple sequence repeat SSR markers including 32 novel markers reported here for the first time on 159 A cytoplasmic male sterile B maintainer and R fertility restorer lines In total 41 277 markers showed polymorphism with 2 to 6 average 26 alleles and 001 to 081 average 034 polymorphism information content PIC value Of these polymorphic markers 22 SSR markers showed polymorphism between A ICPA 2039 and R ICPR 2438 lines of the commercial hybrid ICPH 2438 however only 21 of these SSR markers showed the same profile between A ICPA 2039 and B ICPB 2039 lines Finally two SSR markers CCB4 and CCttc006 were found most suitable for purity assessment of hybrid seeds of the ICPH 2438 hybrid The utility of these two diagnostic SSR markers has been demonstrated by using seed lots of this hybrid from two sources ICRISAT and Mahabeej It is anticipated that molecular diversity information generated on parental lines of hybrids under development and identification of the two most suitable markers for testing the purity of hybrid seeds of ICPH 2438 will facilitate the pigeonpea hybrid breeding programmeThe study was funded by the Pigeonpea Genomics Initiative of the Indian Council of Agricultural Research ICAR under the framework of IndoUS Agricultural Knowledge Initiative AKI Generation Challenge Programme wwwgenerationcporg and Tropical Legume II TL II of Bill and Melinda Gates Foundation BMGF

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