Authors: Jia Guo Yanfeng Zhang Maixia Hui Yongan Cheng Enhui Zhang Zhongmin Xu
Publish Date: 2016/08/04
Volume: 36, Issue: 8, Pages: 117-
Abstract
Recessive genic male sterility RGMS is common in plants and has been widely applied as an effective and economic system for hybrid seed production in many crops However little is known regarding the molecular mechanisms of RGMS in cabbage Brassica oleracea L var capitata due to limited transcriptomic and genomic data Comparative transcriptomic analyses were performed on the sterile RGMS632MS and fertile plants RGMS632SF of a RGMS line RGMS632 using secondgeneration Illumina sequencing to identify critical genes and pathways associated with male sterility A total of approximately 109 million sequencing reads was obtained using RNAseq Abundance analysis identified a total of 5107 unigenes that showed significant differences between the two kinds of plants Among these 1558 genes were upregulated while 3549 genes were downregulated by more than twofold in RGMS632MS compared to RGMS632SF KEGG pathway enrichment analysis revealed changes in the transcript abundance of genes involved in the metabolism and signal transduction of various phytohormones The majority of hormone signalling pathways were downregulated in RGMS632MS Furthermore a set of potential candidate genes involved in the formation or abortion of pollen were investigated These results increased our understanding of the molecular mechanisms and biological processes in RGMS plantsThis study is supported by the National Science and Technology pillar program during the twelfth 5year plan period 2012BAD02B01 the science and technology program of Shaanxi China 2013K0205 and the basic scientific research fund of Northwest AF University Z109021423
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