Authors: Papp Maria Altorjay Istvan Norman Gary L Shums Zakera Palatka Karoly Vitalis Zsuzsanna Foldi Ildiko Lakos Gabriella Tumpek Judit Udvardy Miklos L Harsfalvi Jolan Fischer Simon Lakatos Laszlo Kovacs Agota Bene Laszlo Molnar Tamas Tulassay Zsolt Miheller Pal Veres Gabor Papp Janos Lakatos Peter Laszlo
Publish Date: 2007/08/01
Volume: 13, Issue: 8, Pages: 984-992
Abstract
Maria Papp Istvan Altorjay Gary L Norman Zakera Shums Karoly Palatka Zsuzsanna Vitalis Ildiko Foldi Gabriella Lakos Judit Tumpek Miklos L Udvardy Jolan Harsfalvi Simon Fischer Laszlo Lakatos Agota Kovacs Laszlo Bene Tamas Molnar Zsolt Tulassay Pal Miheller Gabor Veres Janos Papp Peter Laszlo Lakatos Hungarian IBD Study Group Seroreactivity to microbial components in Crohns disease is associated with ileal involvement noninflammatory disease behavior and NOD2/CARD15 genotype but not with risk for surgery in a Hungarian cohort of IBD patients Inflammatory Bowel Diseases Volume 13 Issue 8 1 August 2007 Pages 984–992 https//doiorg/101002/ibd20146Antibodies directed against Saccharomyces cerevisiae ASCA perinuclear components of neutrophils pANCA and porin protein C of Escherichia coli antiOmpC are reported to be associated with disease phenotype and may be of diagnostic importance in inflammatory bowel disease IBD Since limited data are available from Eastern Europe we assessed the above antibodies in Hungarian IBD patientsIn all 653 wellcharacterized unrelated consecutive IBD patients Crohns disease CD 558 m/f 263/295 duration 81 ± 107 years ulcerative colitis UC 95 m/f 44/51 duration 89 ± 98 years and 100 healthy subjects were investigated Sera were assayed for antiOmp and ASCA by enzymelinked immunosorbent assay ELISA and ANCA by indirect immunofluorescence assay IIF TLR4 and NOD2/CARD15 variants were tested by polymerase chain reaction/restriction fragment length polymorphism PCRRFLP Detailed clinical phenotypes were determined by reviewing the medical chartsAntiOmp ASCA and atypical pANCA antibodies were present in 312 593 and 138 of CD 242 137 and 485 of UC patients and in 20 16 and 56 of controls respectively ASCA and antiOmp positivity were associated with increased risk for CD odds ratio ORASCA = 765 95 confidence interval CI 437–134 OROmp = 181 95 CI 108–305 In a logistic regression analysis antiOmp and ASCA were independently associated with ileal and noninflammatory disease but not with a risk for surgery or response to steroids or infliximab A serology dosage effect was also observed ASCA and antiOmp antibodies were associated with NOD2/CARD15 in addition to a gene dosage effect No associations were found in UCSerological markers were useful in the differentiation between CD and UC in an Eastern European IBD cohort Reactivity to microbial components was associated with disease phenotype and NOD2/CARD15 genotype further supporting the role of altered microbial sensing in the pathogenesis of CD
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