Authors: te Velde Anje A de Kort Floor Sterrenburg Ellen Pronk Inge ten Kate Fiebo JW Hommes Daniel W van Deventer Sander JH
Publish Date: 2007/03/01
Volume: 13, Issue: 3, Pages: 325-330
Abstract
Anje A te Velde Floor de Kort Ellen Sterrenburg Inge Pronk Fiebo JW ten Kate Daniel W Hommes Sander JH van Deventer Comparative analysis of colonic gene expression of three experimental colitis models mimicking inflammatory bowel disease Inflammatory Bowel Diseases Volume 13 Issue 3 1 March 2007 Pages 325–330 https//doiorg/101002/ibd20079Using Agilent mouse TOX oligonucleotide microarrays we analyzed colonic gene expression profiles in three widely used models of experimental colitis In 2 of the models TNBS and DSSinduced colitis exogenous agents induce the colitis In the third model the colitis is induced after transfer of a Tcell population CD4+CD45RBhigh T cells that lacks regulatory cells into an immunodeficient hostCompared with control mice in DSS TNBS and the CD45RB transfer colitis mice 387 21 and 582 genes were more than 2fold upregulated in the intestinal mucosa Analyses of exclusively shared gene expression profiles between the different models revealed that DSS/transfer colitis share 69 concordantly upregulated genes DSS/TNBS 6 and TNBS/transfer colitis 1 Seven genes were upregulated in all three models The CD45RB transfer model expression profile included the most genes that are known to be upregulated in IBD Of 32 genes that are known to change transcriptional activity in IBD TNF IFNγ Ltβ IL6 IL16 IL18R1 IL22 CCR2 7 CCL2 3 4 5 7 11 17 20 CXCR3 CXCL1 5 10 Mmp3 79 14 Timp1 Reg3γ and Pap S100a8 S100a9 Abcb1 and Ptgs2 2/32 are upregulated in TNBS 15/32 are upregulated or downregulated in DSS and 30/32 are upregulated or downregulated in the CD45RB transfer colitis
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