Authors: Willing Ben Halfvarson Jonas Dicksved Johan Rosenquist Magnus Järnerot Gunnar Engstrand Lars Tysk Curt Jansson Janet K
Publish Date: 2009/05/01
Volume: 15, Issue: 5, Pages: 653-660
Abstract
Ben Willing Jonas Halfvarson Johan Dicksved Magnus Rosenquist Gunnar Järnerot Lars Engstrand Curt Tysk Janet K Jansson Twin Studies Reveal Specific Imbalances in the Mucosaassociated Microbiota of Patients with Ileal Crohns Disease Inflammatory Bowel Diseases Volume 15 Issue 5 1 May 2009 Pages 653–660 https//doiorg/101002/ibd20783To reduce variations in exposure during establishment of the gut flora and the influence of genotype we studied the mucosaassociated microbiota of monozygotic twin pairs that were discordant n = 6 or concordant n = 4 for CD DNA was extracted from biopsies collected from 5 locations between the ileum and rectum Bacterial 16S ribosomal RNA genes were amplified and community composition assessed by terminalrestriction fragment length polymorphism cloning and sequencing and quantitative realtime polymerase chain reaction PCRThe microbial compositions at all biopsy locations for each individual were similar regardless of disease state but there were differences between individuals In particular individuals with predominantly ileal CD had a dramatically lower abundance P 0001 of Faecalibacterium prausnitzii and increased abundance P 003 of Escherichia coli compared to healthy cotwins and those with CD localized in the colon This dysbiosis was significantly correlated to the disease phenotype rather than genotypeThe reduced abundance of F prausnitzii and increased abundance of E coli are indicative of an ileal CD phenotype distinct from colonic CD and the relative abundances of these specific bacterial populations are promising biomarker candidates for differential diagnosis of CD and eventually customized treatment
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