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Title of Journal: Chin Sci Bull

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Abbravation: Chinese Science Bulletin

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SP Science in China Press

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10.1016/j.ajic.2004.04.018

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1861-9541

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Increased osteoblast adhesion on nanophase Ti6Al4V

Authors: WeiPing Ji Pei Han ChangLi Zhao Yao Jiang XiaoNong Zhang
Publish Date: 2008/06/05
Volume: 53, Issue: 11, Pages: 1757-1762
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Abstract

The objective of the present study was to prepare a novel nanostructured surface of Ti6Al4V alloy by the severe plastic deformation SPD and the chemical treatment process and to evaluate the adhesion of osteoblast on the nanophase titanium alloy In the in vitro study the primary cultured osteoblasts of neonatal rat calvaria were cultured on the nanophase and the asreceived smooth Ti6Al4V substrates Then osteoblasts adhesion behaviors on different substrates were observed by the fluorescence microscopy scanning electron microscopy SEM and RTPCR analysis The results of our research showed increased osteoblast adhesion on the nanophase titanium alloy compared with the asreceived case On the nanophase substrate the presence of extensive filopodia strong cellular adhesion and early cellular confluency could be observed In addition the expression of the adhesionrelated integrin β1 mRNA was also higher on the nanophase substrate It suggested that the nano technology could be further considered for orthopedic implant applications

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