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Title of Journal: Chin Sci Bull

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Abbravation: Chinese Science Bulletin

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SP Science China Press

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DOI

10.1016/0272-8842(85)90181-6

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1861-9541

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Aromatase and steroid sulfatase mRNA expression in

Authors: Lei Gao Ying Qi JingLi Liu XiaoMeng Yu Wei Xia GuiJian Liu
Publish Date: 2012/07/01
Volume: 57, Issue: 20, Pages: 2574-2579
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Abstract

The fine needle aspiration FNA test is a convenient and tolerable technique with minimal invasion that is accepted by most women Local estrogen synthesis depends mainly on the aromatase and steroid sulfatase pathways that are believed to play important roles in breast carcinogenesis However little is known about the level of aromatase and steroid sulfatase mRNA expression in FNA samples which contain only small amounts of tissue The nested QPCR assay has been proven to be a highly sensitive and specific method to assess the aromatase expression of breast tissue In this study aromatase and steroid sulfatase mRNA expression in 74 patients with benign or malignant disorders was evaluated and compared using nested QPCR and nonnested QPCR assays The expression levels were analyzed and correlated with clinical parameters No difference in the aromatase expression levels between nested and nonnested QPCR was noticed Age and aromatase mRNA expression level were two independent risk factors for breast cancer P=004 and P=000 respectively while menopausal status and steroid sulfatase mRNA expression levels were not associated with breast cancer This study showed that both nested and nonnested QPCR assays were effective methods for research using FNA breast samplesThis article is published under an open access license Please check the Copyright Information section for details of this license and what reuse is permitted If your intended use exceeds what is permitted by the license or if you are unable to locate the licence and reuse information please contact the Rights and Permissions team

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