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Title of Journal: Chin Sci Bull

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Abbravation: Chinese Science Bulletin

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SP Science in China Press

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DOI

10.1002/nme.1620231106

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1861-9541

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Differential gene expression profiling in the deve

Authors: Pu Yang WenWu Zhou Qin Zhang JiaAn Cheng ZengRong Zhu M O Way
Publish Date: 2009/11/10
Volume: 54, Issue: 20, Pages: 3822-
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Abstract

The rice water weevil Lissorhoptrus oryzophilus Kuschel Coleoptera Curculionidae reproduces by sex in the Southeastern United States but reproduces by parthenogenesis in California and other invaded regions in Asia and Europe The objective of this study was to create a parthenogenetic gene expression profile of the rice water weevil in order to gain a better insight into the molecular mechanisms of parthenogenesis in the weevil Suppression subtractive hybridization SSH technique was employed for profiling differential gene expression in the developed ovary between the parthenogenetic and bisexual female rice water weevils A total of 70 contigs were obtained and the BLASTX search identified putatively 28 genes with differential functions According to the cytological process of parthenogenesis the tubulin alpha1 chain and signal transduction genes etc were selected for real time quantitative RTPCR analyses and their possible functions related to the molecular mechanism of parthenogenesis were discussed The tubulin alpha1 chain and some signal transduction genes may be related to the molecular mechanisms of parthenogenesis of the rice water weevilSupported by the National Key Basic Research and Development Program of China Grant No 2009CB119200 National High Technology Research and Development Program of China Grant No 2007AA10Z220 and Key Projects in the National Science Technology Pillar Program in the Eleventh Fiveyear Plan Period Grant No 2006BAD17B06


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