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Title of Journal: Chin Sci Bull

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Abbravation: Chinese Science Bulletin

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SP Science China Press

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DOI

10.1016/0002-9378(88)90582-0

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1861-9541

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Complete sequence and gene organization of the mit

Authors: ChengYe Wang Ying Feng XiaoMing Chen
Publish Date: 2012/09/07
Volume: 57, Issue: 27, Pages: 3578-3585
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Abstract

Batocera lineolata Chevrolat 1852 is an important forest pest that is found mainly in China Vietnam India and Japan The complete mitochondrial genome mitogenome sequence of B lineolata was determined by long polymerase chain reaction PCR and conserved primer walking approaches The results showed that the entire mitogenome is 15418 bp long with 7448 A+T content The positions and arrangement of the 37 genes encoded by the genome are identical to the mitogenomes of two other longhorn beetles for which the complete gene content and arrangement are publicly available All proteincoding genes start with the ATN codon that is a typical initiation codon in insects All transfer RNAs tRNAs were predicted to form the standard cloverleaf structure except for tRNA Ser AGN which lacks the dihydrouridine DHU arm The most unusual feature that was found was the use of TCT as the tRNA Ser AGN anticodon instead of the GCT that is used in most other arthropods The lack of tandem repeat motif in the 735 bp long A+Trich region was another unusual feature of the B lineolata mitogenome The short highly conserved polythymidine stretch that was previously described in the Orthoptera and Diptera orders was also present in the A+Trich region of the B lineolata order Coleoptera mitogenome The sequence and annotation of the mitogenome of B lineolata will provide further insights into the diversity and evolution of the Cerambycidae family of longhorned beetles The B lineolata mitogenome sequence has been deposited in GenBank http//wwwncbinlmnihgov/Genbank/ under accession number JN986793This article is published under an open access license Please check the Copyright Information section for details of this license and what reuse is permitted If your intended use exceeds what is permitted by the license or if you are unable to locate the licence and reuse information please contact the Rights and Permissions team

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