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Title of Journal: Food Anal Methods

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Abbravation: Food Analytical Methods

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Springer-Verlag

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1936-976X

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Statistical Evaluation of RealTime PCR Protocols

Authors: Silvia Folloni Gianni Bellocchi Adelina Prospero Maddalena Querci William Moens Monica Ermolli Guy Van den Eede
Publish Date: 2010/03/30
Volume: 3, Issue: 4, Pages: 304-312
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Abstract

Realtime PCR RTiPCR is the technique of choice for eventspecific quantification of genetically modified organisms GMOs by determining the amount of event with respect to a speciesspecific reference gene Reference genes can be amplified from the genome extracted from Certified Reference Materials CRMs or from ad hoc designed plasmids In the present study we statistically evaluate the performance of RTiPCR protocols for GM maize MON810 event by using both genomic DNA from conventional CRMs and a plasmid containing sequences representative of four maize speciesspecific reference genes The significance of simple and interaction effects of several variables included in the experimental design on DNA quantification methods and RTiPCR were evaluated and discussed Statistically significant differences on Ct values may have an impact on the GMOs quantification and consequently on the compliance of GM quantificationestablished legal thresholds Our results confirm the reliability of the plasmid as alternative calibrant for the calculation of GMOs copy number

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Other Papers In This Journal:
  1. High-Throughput ICP-MS and Chemometrics for Exploring the Major and Trace Element Profile of the Mediterranean Sepia Ink
  2. Selenium Speciation in Rice Samples by Magnetic Ionic Liquid-Based Up-and-Down-Shaker-Assisted Dispersive Liquid-Liquid Microextraction Coupled to Graphite Furnace Atomic Absorption Spectrometry
  3. Improvement of Energy Dispersive X-Ray Fluorescence Throughput: Influence of Measuring Times and Number of Replicates on Validation Performance Characteristics
  4. Direct Determination of Saccharin and Acesulfame-K in Sweeteners and Fruit Juices Powders
  5. Electrochemical Determination of Carbendazim in Food Samples Using an Electrochemically Reduced Nitrogen-Doped Graphene Oxide-Modified Glassy Carbon Electrode
  6. Simultaneous Determination of Caffeine and Aspartame in Diet Supplements and Non-Alcoholic Beverages Using Liquid-Chromatography Coupled to Corona CAD and UV-DAD Detectors
  7. Recovery of Aromatic Aglycones from Grape Pomace Winemaking By-Products by Using Liquid-Liquid and Pressurized-Liquid Extraction
  8. Does the Plant Matrix Type Have Impact on Rutin Transformation During Its Extraction?
  9. An Enzymatic Method for Methanol Quantification in Methanol/Ethanol Mixtures with a Microtiter Plate Fluorometer
  10. Determination of Mineral Composition and Heavy Metal Content of Some Nutraceutically Valued Plant Products
  11. Evaluation and Optimization of a Superior Extraction Method for the Characterization of the Volatile Profile of Black Tea by HS-SPME/GC-MS
  12. Determination of Zearalanol and Its Analog Zearalanone in Muscle Tissues by Amplified Luminescent Proximity Homogeneous Assay (AlphaLISA)
  13. Validation of a HS-SPME-GC Method for Determining Higher Fatty Esters and Oak Lactones in White Rums
  14. Development of a Single Chain Variable Fragment Antibody and Application as Amatoxin Recognition Molecule in Surface Plasmon Resonance Sensors
  15. A Low-Cost and High Sensitive Paper-Based Microfluidic Device for Rapid Detection of Glucose in Fruit
  16. A Selective Chromatographic Method to Determine the Dynamic of Biogenic Amines During Brewing Process
  17. Validation of HPLC-UV Methods for the Quantification of Betaine in Foods by Comparison with LC-MS
  18. On-Vine Monitoring of Grape Ripening Using Near-Infrared Spectroscopy
  19. Detection Method Optimization, Dynamic Changes during Alcoholic Fermentation and Content Analysis of “Brett Character” Compounds 4-Ethylphenol (4-EP) and 4-Ethylguaiacol (4-EG) in Chinese Red Wines
  20. Rapid and Non-destructive Determination of Moisture Content of Peanut Kernels Using Hyperspectral Imaging Technique
  21. Analysis of Free Amino Acids in Russula griseocarnosa Harvested at Different Stages of Maturity Using iTRAQ ® -LC-MS/MS
  22. Development and Validation of a Cost-Effective HPLC-FLD Method for Routine Analysis of Fumonisins B 1 and B 2 in Corn and Corn Products
  23. Development and Validation of a Cost-Effective HPLC-FLD Method for Routine Analysis of Fumonisins B 1 and B 2 in Corn and Corn Products
  24. Determination of Soluble Calcium and Phosphorus in Commercial Milled Hydroxyapatite
  25. Rapid Determination of Tetracyclines Hydrochloride Using ATR FT-MIR Spectroscopy
  26. Analysis of Pesticide Residues and Their Variability in Cabbage Using QuEChERS Extraction in Combination with LC-MS/MS
  27. Provision of Ultrasensitive Quantitative Gold Immunochromatography for Rapid Monitoring of Olaquindox in Animal Feed and Water Samples
  28. The Mesoporous Porphyrinic Zirconium Metal-Organic Framework for Pipette-Tip Solid-Phase Extraction of Mercury from Fish Samples Followed by Cold Vapor Atomic Absorption Spectrometric Determination
  29. Analysis of Pesticide Residues and Their Variability in Cabbage Using QuEChERS Extraction in Combination with LC-MS/MS
  30. Optimized Formation, Extraction, and Determination of Sulforaphane in Broccoli by Liquid Chromatography with Diode Array Detection
  31. Determination of Haloxyfop-Methyl, Linuron, and Procymidone Pesticides in Carrot Using SLE-LTP Extraction and GC-MS
  32. Determination of Aflatoxins M1, M2, B1, B2, G1, and G2 and Ochratoxin A in UHT and Powdered Milk by Modified QuEChERS Method and Ultra-High-Performance Liquid Chromatography Tandem Mass Spectrometry
  33. Preparation of Molecularly Imprinted Polymer Monolith with an Analogue of Thiamphenicol and Application to Selective Solid-Phase Microextraction
  34. A Rapid and Sensitive Method for Semicarbazide Screening in Foodstuffs by HPLC with Fluorescence Detection
  35. Evaluation of Extraction Methods for Efficient Detection of Enteric Viruses in Pork Meat Products
  36. Optimization and Application of a HS-SPME-GC-MS Methodology for Quantification of Furanic Compounds in Espresso Coffee
  37. Liquid Chromatography for the Rapid Screening of Growth Promoters Residues in Meat
  38. Direct Determination of Molybdenum in Milk and Infant Food Samples Using Slurry Sampling and Graphite Furnace Atomic Absorption Spectrometry
  39. Improvement of the Detection Sensitivity for Staphylococcus aureus in Spices and Herbs
  40. Ionic Liquid-based Ultrasound-Assisted In Situ Solvent Formation Microextraction Combined with Electrothermal Atomic Absorption Spectrometry as a Practical Method for Preconcentration and Trace Determination of Vanadium in Water and Food Samples
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