Authors: André Victor Sartori Juliana Swensson de Mattos Maria Heloísa Paulino de Moraes Armi Wanderley da Nóbrega
Publish Date: 2015/02/19
Volume: 8, Issue: 9, Pages: 2321-2330
Abstract
A suitable method for routine analysis of aflatoxins M1 M2 B1 B2 G1 and G2 and ochratoxin A in milk by modified Quick Easy Cheap Effective Rugged and Safe QuEChERS sample treatment method and ultrahighperformance liquid chromatography tandem mass spectrometry UHPLCMS/MS was developed and validated Satisfactory recoveries ranging from 72 to 121 with relative standard deviation lower than 17 were obtained for the target compounds The limits of quantification for aflatoxin M1 were 0017 and 025 μg kg−1 for ultrahightemperature UHT milk and powdered milk respectively The validated method was successfully applied to the determination of mycotoxins in milk samples from local supermarkets in Rio de Janeiro Brazil Ochratoxin A and aflatoxins B1 B2 G1 and G2 were not detected in the analyzed samples Aflatoxin M1 was found in 53 74 of the powdered milk samples analyzed at concentration levels ranging from 008 to 119 μg kg−1 In UHT milk aflatoxin M1 was found in 11 69 of the analyzed samples at concentration levels ranging from 0005 to 0042 μg kg−1 The concentrations found for aflatoxin M1 in milk samples were lower than the maximum permitted level according to Brazilian regulation Aflatoxin M2 was detected in 17 24 of the powdered milk samples 008 μg kg−1 and in 3 18 of the UHT milk samples 0009 μg kg−1 The present study is the first report showing the incidence of aflatoxin M2 in milk in BrazilAndré Victor Sartori declares that he has no conflict of interest Juliana Swensson de Mattos declares that she has no conflict of interest Maria Heloísa Paulino de Moraes declares that she has no conflict of interest Armi Wanderley da Nóbrega declares that he has no conflict of interest This article does not contain any studies with human or animal subjects
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