Authors: Mohammed Hefni Christopher McEntyre Michael Lever Sandy Slow
Publish Date: 2015/05/17
Volume: 9, Issue: 2, Pages: 292-299
Abstract
The importance of dietary betaine is increasingly recognized The aim of this study was to develop a simple highperformance liquid chromatography with standard ultraviolet spectrometric detection HPLCUV method for betaine NNNtrimethylglycine determination in foods after derivatization Two methods were used for betaine derivatization Thereafter derivatized betaine was quantified using HPLCUV and the results were compared with liquid chromatography mass spectrometry LCMS The established derivatizing agent 2′naphthacyl triflate and a novel derivatizing agent 2bromo2′acetonaphthone produced the same cationic derivative when they react with betaine The calibration curves were linear up to 1000 μmol/L R 2 = 09974 for 2′naphthacyl triflate and 09995 for 2bromo2′acetonaphthone The limit of detection was 1 μmol/L for both methods 2′naphthacyl triflate and 2bromo2′acetonaphthone confirming sufficient sensitivity for betaine quantification in foods The average recovery from different food matrices wheat flour and spinach n = 12 was 99 ± 9 95 ± 10 and 101 ± 8 for LCMS 2′naphthacyl triflate and 2bromo2′acetonaphthone respectively Inter and intraassay coefficients of variation CVs in the control samples whole wheat flour were below 10 Quantitative results for foods analyzed using 2′naphthacyl triflate and 2bromo2′acetonaphthone were comparable to LCMS R 2 = 0992 and 0990 respectively The highest betaine content ~160 mg/100 g was found in spinach followed by faba bean wheat flour and beetroot These methods can be widely used for betaine quantification because of the simplicity of the derivatization procedures and the commercial availability of the derivatizing reagent 2bromo2′acetonaphthone or through the relatively easy synthesis of 2naphthacyl triflateScholarship funding from the Partner Ownership initiative program ParOwn grant number 20150 the Ministry of Higher Education Egypt is gratefully acknowledged Assistance from the National Heart Foundation of New Zealand and from the Maurice Phyllis Paykel Trust is also acknowledgedMohammed Hefni declares that he has no conflict of interest Christopher McEntyre declares he has no conflict of interest Michael Lever declares that he has no conflict of interest Sandy Slow declares she has no conflict of interest This article does not contain any studies with human or animal subjects
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