Authors: Ana M Ares José Bernal María T Martín José L Bernal María J Nozal
Publish Date: 2013/11/30
Volume: 7, Issue: 3, Pages: 730-740
Abstract
A new method has been developed to determine sulforaphane in broccoli florets stems and leaves using liquid chromatography coupled to diode array detection The hydrolysis reaction required to convert glucoraphanin to its breakdown product sulforaphane and the extraction procedure based on solvent extraction with methyl tbutyl ether followed by solid phase extraction with silica cartridges were optimized for the three broccoli matrices the resulting recovery values ranged from 92 to 102 in all cases A fast separation was performed on a C18 analytical column with a mobile phase composed of ammonium formate 20 mM in water and acetonitrile 5545 v/v in isocratic elution mode This method was fully validated and was found to be selective linear from 25 to 800 mg/kg and precise percent relative standard deviation values below 5 Moreover the limits of detection and quantification were 08 and 25 mg/kg respectively Finally the proposed method has been applied to the analysis of sulforaphane in broccoli samples of two different cultivars Parthenon and Marathon
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