Authors: Tereza Cabicarová Eva Kaclíková Anneluise Mader Jana Minarovičová Janka Koreňová Tomáš Kuchta
Publish Date: 2015/12/17
Volume: 9, Issue: 7, Pages: 1980-1984
Abstract
Various variants of ISO 688811999 and ISO 688832003 methods for the detection of Staphylococcus aureus were evaluated and improved for their application to conduct analysis in spices and herbs Improvement substantiated in washing of the sample to remove compounds interfering with the analysis and in the use of PCR for final detection instead of plating on BairdParker agar to improve sensitivity at high backgrounds and to save time The evaluation of the method variants was based on determination of the detection limit LOD using series of artificially contaminated spices allspice black pepper cinnamon nutmeg paprika vanilla and herbs basil oregano parsley thyme The method without enrichment ISO 688811999 produced LODs of 103–105 CFU/g with no positive effect of washing the sample or use of PCR for final detection The method with enrichment ISO 688832003 had LOD of 100 CFU/g for basil black pepper paprika and parsley If the washing step was added and PCR was used for final detection LOD of 100 CFU/g was determined also for cinnamon nutmeg and vanilla and LOD of 101 CFU/g was determined for allspice For oregano and thyme which strongly inhibit the growth of S aureus an alternative enrichmentindependent method based on direct DNA extraction coupled to realtime PCR may be advantageousThis research was carried out in frames of the FP7SEC20121 project “Securing the spices and herbs commodity chains against deliberate accidental or natural biological and chemical contamination SPICED No 312631” The authors thank to Dr V Janská and Dr K Ženišová for valuable advice on methodologyTereza Cabicarová declares that she has no conflict of interest Eva Kaclíková declares that she has no conflict of interest Anneluise Mader declares that she has no conflict of interest Jana Minarovičová declares that she has no conflict of interest Janka Koreňová declares that she has no conflict of interest Tomáš Kuchta declares that he has no conflict of interest
Keywords: