Authors: Guoliang Li Chenhong Tang Ying Wang Jing Yang Hongliang Wu Guang Chen Xiaojian Kong Weiheng Kong Shucheng Liu Jinmao You
Publish Date: 2014/12/17
Volume: 8, Issue: 7, Pages: 1804-1811
Abstract
Semicarbazide SEM has been proven to extensively exist in foodstuffs due to anthropogenic factor in food processing and possesses various toxic effects on human health Although many methods have been developed they often require long analytical time complex laboratory equipment trained personnel difficultly prepared antibodies or relatively expensive equipment The present study developed a new method for SEM determination by HPLC with fluorescence detection FLD The fluorescence reagent 211Hbenzoacarbazol11yl ethyl chloroformate BCEC was first used for SEM labeling The fluorescent labeling conditions were optimized systematically SEM can be labeled in only 10 min at 40 °C The labeled SEM was analyzed on an eclipse XDBC8 column in 8 min The new method offered the low LOD of 04 μg/kg at a signaltonoise ratio of 3 and also exhibited excellent reproducibility precision and accuracy When applied to analyze several foodstuffs it showed good applicability The developed method has been proven to be simple inexpensive selective sensitive accurate and reliable for SEM analysis in foodstuffs Furthermore this developed method should have a powerful potential in the analysis of SEM from many other food samplesThis work was supported by The National Natural Science Foundation of China No 31301595 21475075 21475074 and 21275089 the Open Project Program of Guangdong Provincial Key Laboratory of Aquatic Products Processing and Safety Guangdong Ocean University GDPKLAPPS1401 PhD research startup funds of Qufu Normal University bsqd 2012017 and the Soft Science Project of Shandong Province 2013RKA08020Guoliang Li declares that he has no conflict of interest Chenhong Tang declares that he has no conflict of interest Ying Wang declares that she has no conflict of interest Jing Yang declares that she has no conflict of interest Hongliang Wu declares that he has no conflict of interest Guang Chen declares that he has no conflict of interest Weiheng Kong declares that he has no conflict of interest Xiaojian Kong declares that he has no conflict of interest Shucheng Liu declares that he has no conflict of interest Jinmao You declares that he has no conflict of interest
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