Authors: Oscar Galarce Karem HenríquezAedo Darlene Peterssen Carlos PeñaFarfal Mario Aranda
Publish Date: 2016/05/13
Volume: 9, Issue: 12, Pages: 3385-3395
Abstract
A highly selective method was established to study the dynamic of biogenic amine formation during the brewing process The presence of histamine tyramine cadaverine putrescine phenylethylamine spermidine and spermine was determined in raw materials barley hop yeast intermediate products sweet wort hopped wort green and matured beer and beers Dansylated amines were separated on a ZorbaxXDB C18 column using a binary mobile phase composed of acetonitrile and water Chromatographic conditions were optimized using an experimental design and validation was performed following International Conference on Harmonization ICH recommendations Calibration data fitted a linear regression model with R 2 099 Repeatability n = 6 and intermediate precision n = 3 in matrix showed relative standard deviation RSD values lower than 762 and 851 respectively Recoveries at three different concentrations ranged from 7397 to 11494 The proposed method was applied to determine the dynamic of biogenic amine content during brewing process of three microbreweries allowing the detection of the principal sources or stages of biogenic amine BA contribution/formationThis work is part of Oscar Galarce thesis to obtain the degree of Magister in Pharmaceutical Sciences from the University of Concepcion National Fund for Scientific and Technological Development FONDECYT project no 1131080 Fund for Scientific and Technological Equipment FONDEQUIP project no 130209 and the University of Concepcion financially supported this work
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