Authors: Xingyao Pei Qi Wang Xiangmei Li Jie Xie Sanlei Xie Tao Peng Cheng Wang Yuanze Sun Haiyang Jiang
Publish Date: 2015/11/28
Volume: 9, Issue: 7, Pages: 1919-1927
Abstract
Olaquindox OLA used as a medicinal feed additive has been put under ban due to hazard concerns over animalderived food security In this study a simple rapid ultrasensitive and quantitative gold immunochromatography assay GICA was established to analyze OLA in animal feed samples and surface water samples to monitor food security Various trying has been experimented to improve the sensitivity The IC50 of the optimized method is 335 μg L−1 for feedstuff and 035 μg L−1 for environmental water The recoveries ranged from 7733 to 8691 CV 2362 for spiked feedstuff and 9630 to 11783 CV 2251 for spiked water samples Then the developed GICA was applied to animal feed and field water followed by confirmation with ELISA and the consistency of results indicated that the developed GICA could be applied for rapid screening of OLA in real samples Compared with previous assay the developed GICA in this study was more sensitive and more rapid which exhibited broader prospect to supervise the animal products and water rapidlyThis study was supported by the Special Research Project of Public Welfare Quality Testing Industry 2012104003 and the National Science Technology Pillar Program during the 12th 5year Plan Period 2015BAK36B03 And the authors appreciate the cooperation of other faculty members in the Department of Pharmacology and Toxicology of the College of Veterinary Medicine at China Agricultural UniversityXingyao Pei declares that she has no conflict of interest Qi Wang declares that she has no conflict of interest Xiangmei Li declares that she has no conflict of interest Jie Xie declares that she has no conflict of interest Sanlei Xie declares that he has no conflict of interest Tao Peng declares that he has no conflict of interest Cheng Wang declares that he has no conflict of interest Yuanze Sun declares that she has no conflict of interest Haiyang Jiang declares that he has no conflict of interest
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