Authors: Chao Ma Hui Chen Na Sun Yong Ye Huaixia Chen
Publish Date: 2012/02/21
Volume: 5, Issue: 6, Pages: 1267-1275
Abstract
A molecularly imprinted polymer MIP monolith has been prepared and characterized Its application to the assay of thiamphenicol in milk with highperformance liquid chromatography–photodiodes array detector was validated The newly developed MIP monolith was produced using an analogue to thiamphenicol as the template molecule to avoid major traditional drawback associated with MIPs of residual template bleeding The MIP monolith synthesized in a micropipette tip could be connected with syringes in different sizes simply to perform solidphase microextraction process without any other treatment This molecularly imprinted polymer monolith microextraction MIPMME method showed high selectivity and enrichment ability for thiamphenicol TAP Several parameters affecting MIPMME were investigated including the flow rate volume pH and salt concentration of sample the type and volume of washing solution and the type and flow rate of eluent The recovery of this method for TAP was investigated and high recoveries of 935 ~ 968 from milk were obtained with relative standard deviations less than 63This work was supported by Education Department of Hubei Province grant no T201101 the National Nature Science Foundation of China grant no 20975030 20835004 the Natural Science Foundation of Hubei province of China grant no 2009CDB364 and the Specialist Fund of Hubei University 020091130ky2006004 The authors would like to thank their colleagues for their valuable technical assistance
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