Authors: Jianyuan Qin Lijuan Xie Yibin Ying
Publish Date: 2016/03/17
Volume: 9, Issue: 10, Pages: 2880-2886
Abstract
Antibiotic residues in animalderived foods have brought serious threats to human health as well as economic losses to the food industry Given that analytical methods are crucial but remain limited a rapid reliable and costeffective method is needed for detecting antibiotic residues In this paper a method using attenuated total reflection Fourier transform midinfrared ATR FTMIR spectroscopy combined with chemometrics was established for the detection of tetracyclines hydrochloride TCsH Firstly TCsH powders were scanned using ATR FTMIR spectroscopy and the characteristic peaks of these samples were found in this region Then milk samples with different TCsH concentrations 1 to 160 ppb were measured and were analyzed using principal component analysis PCA and partial least squares regression PLSR algorithms The results showed that the kinds of TCsH in milk could not be classified However TCsH concentration ranging from 1 to 160 ppb in milk samples was successfully determined with high determination coefficient R 2 values of 088–090 low rootmeansquare errors values of 976–182 and high residual predictive deviation RPD values close to or greater than 3 These results indicated that ATR FTMIR spectroscopy could be used to identify TCsH powders based on their unique spectral features and was suitable for the rapid detection of TCsH concentration in milk
Keywords: