Authors: G Yi SK Lee YK Hong YC Cho MH Nam SC Kim SS Han GL Wang TR Hahn PC Ronald JS Jeon
Publish Date: 2004/05/13
Volume: 109, Issue: 5, Pages: 978-985
Abstract
Identification of the PCR markers tightly linked to genes that encode important agronomic traits is useful for markerassisted selection MAS The rice Pi5t locus confers broadspectrum resistance to Magnaporthe grisea the causal agent of rice blast disease It has been hypothesized that the Pi5t locus carries the same gene as that encoded by the Pi3t and Piit loci We developed three PCRbased dominant markers JJ80T3 JJ81T3 and JJ113T3 from three previously identified BIBAC clones—JJ80 JJ81 and JJ113—that are linked to the Pi5t locus PCR analysis of 24 monogenic lines revealed that these markers are present only in lines that carry Pi5t Pi3t and Piit PCR and DNA gelblot analysis of candidate resistance lines using JJ80T3 JJ81T3 and JJ113T3 indicated that Tetep is the likely donor of Pi5t Of the 184 rice varieties tested 34 carried the JJ80T3 JJ81T3 and JJ113T3specific bands Disease evaluation of those 34 varieties revealed that all conferred resistance to PO66 The genomic structure of three of these resistant varieties ie IR72 Taebaeg Jahyangdo is most similar to that of Pi5t Our results demonstrate the usefulness of the JJ80T3 JJ81T3 and JJ113T3 markers for MAS for M grisea resistanceWe thank Dave Mackill SangNak Ahn SangWon Ahn Kenong Xu Tsuyoshi Inukai Dahu Chen Kangle Zheng and Matt Campbell for helpful discussions and Harold Bockelman for providing C104PKT LAC23 PKT and Tetep We also thank Priscilla Licht for critical reading of the manuscript This work was supported in part by grants from the Biogreen 21 Program Rural Development Administration from SRC for the Plant Metabolism Research Center PMRC Korea Science and Engineering Foundation KOSEF Program from the BK21 program Ministry of Education and from the United States Department of Agriculture
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