Authors: Mingjie Chen Mark Wilkinson Paola Tosi Guangyuan He Peter Shewry
Publish Date: 2005/08/17
Volume: 111, Issue: 6, Pages: 1159-1166
Abstract
DNA from six hexaploid tetraploid and diploid species of Aegilops with the C D S M and U genomes was amplified with specific PCR primers to identify sequences encoding puroindolines Pins a and b and grain softness protein GSP all of which are encoded by genes at the Ha hardness locus with Ae tauschii DD and bread wheat T aestivum AABBDD cv Hiline being studied as controls Seven new allelic forms of Pin a and Pin b were identified including forms with mutations within or close to the tryptophan motif In addition five new forms of GSP were detected In all species both genomic DNA from leaves and cDNA from developing grain were analysed This revealed the presence of both silent genes with premature stop codons and multiple genes with the latter being confirmed by Southern blot analysis Freeze fracture analysis demonstrated that all except one accession Ae sharonensis were soft textured However this difference cannot be accounted for by the sequences of the Pin alleles present in this lineRothamsted Research receives grantaided support from the Biotechnology and Biological Sciences Research Council of the United Kingdom We wish to thank Rothamsted International for providing a Fellowship for M Chen and Dr Nigel Halford for assistance with Table 3 We are also very grateful to Dr Huw Jones and Ms Caroline Sparks for advice and discussions and to Mrs Jean Devonshire for her assistance with the SEM
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